There are three concepts which include immunohistochemistry, immunocytochemistry and immunofluorescence. The assay of immunohistochemistry is relatively easy to confirm and distinguish from other two assays. However, what are the assay of immunocytochemistry and immunofluorescence? How to different the assay of immunocytochemistry and immunofluorescence? When we write the research article to be published in magazine, which one word should be exactly word to use?
From the scope of the concept, the range of coverage of immunofluorescence its larger and immunofluorescence include immunohistochemistry and immunocytochemistry. Let us put it another way,immunohistochemistry and immunocytochemistry are one type of immunofluorescence.
Immunohistochemistry is an assay for confirming the expression and expression location of proteins in nature tissue section. Immunofluorescence can be used on cultured cell lines, tissue sections, or individual cells. Immunofluorescence may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules in cells or tissues.Immunocytochemistry is performed on sample of intact cells.
Immunocytochemistry (ICC) is a common laboratory assay that can confirm the expression and location of target peptides or protein antigens in the cell via specific combination of antibodies and target molecules. These bound antibodies can then be detected using several different methods. Immunocytochemistry (ICC) allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen.
There are also two different immunocytochemistry assay which include indirect nd direct immunocytochemistry technique.For indirect immunocytochemistry assay, the protocol mainly include preparation and culture of cells, cells fixation, serum blocking, primary antibody incubation, marked second antibody incubation, staining, result judgment and imaging. For direct immunocytochemistry assay, there are only marked primary antibody been incubated without second antibody and other steps are same with indirect immunocytochemistry.