Anti-CD5 Antibody

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Anti-CD5 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-CD5 Antibody
经验证的应用
WB,ELISA,IHC-P
交叉反应
Reacts with: Human
特异性
Human CD5
免疫原
Recombinant Human CD5 / Cluster of Differentiation 5 Protein (Catalog#11027-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human CD5 / Cluster of Differentiation 5 (rh CD5 / Cluster of Differentiation 5; Catalog#11027-H08H; NP_055022.2; Met1-Pro372). CD5 / Cluster of Differentiation 5 specific IgG was purified by Human CD5 / Cluster of Differentiation 5 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-CD5 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ELISA 1:5000-1:10000
IHC-P 1:1000-1:5000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-CD5 Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining of human CD5 in human tonsil with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human CD5 in human spleen with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Anti-CD5 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:68 kDa

Anti-CD5 Antibody: 别称

Anti-LEU1 Antibody; Anti-T1 Antibody

CD5 背景信息

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.
全称
CD5 molecule
参考文献
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12.
  • Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.
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