CD5L Matched ELISA Antibody Pair Set,Mouse
Cat: SEK50020
CD5L Matched ELISA Antibody Pair Set,Mouse 产品信息
产品名称
CD5L Matched ELISA Antibody Pair Set,Mouse
检测原理
Solid Phase Sandwich ELISA
特异性
Quantitative determination of Mouse CD5L
线性范围
6.25-400 pg/mL
ELISA 抗体对套装（非即用型）组分
1. Capture Antibody 0.5 mg/mL of rabbit anti-mouse CD5L monoclonal antibody, Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 50020-R054)
2. Detection Antibody 0.25 mg/mL rabbit anti-mouse CD5L polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 30 ng of recombinant mouse CD5L. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
2. Detection Antibody 0.25 mg/mL rabbit anti-mouse CD5L polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use.
3. Standard Each vial contains 30 ng of recombinant mouse CD5L. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 400 pg/mL is recommended.
产品概述
This CD5L Matched ELISA Antibody Pair Set,Mouse is a solid phase sandwich ELISA for quantitative determination of Mouse CD5L . It contains Mouse CD5L capture antibody, Mouse CD5L detector antibody
(HRP)
and a highly purified
HEK293-expressed
recombinant Mouse CD5L protein. This Pair Set is at affordable price for researchers.
运输方式
This Matched ELISA Antibody Pair Set is shipped at ambient temperature.
储存条件
Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
Detection Antibody: Protect it from prolonged exposure to light. Aliquot and store at -20℃ to -80℃ and for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.
Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
CD5L Matched ELISA Antibody Pair Set,Mouse 图片
CD5L Matched ELISA Antibody Pair Set,Mouse: 别称
42010 Matched ELISA Antibody Pair Set, Mouse; AAC-11 Matched ELISA Antibody Pair Set, Mouse; AI047839 Matched ELISA Antibody Pair Set, Mouse; Api6 Matched ELISA Antibody Pair Set, Mouse; CT2 Matched ELISA Antibody Pair Set, Mouse; Pdp Matched ELISA Antibody Pair Set, Mouse; Sp-alpha Matched ELISA Antibody Pair Set, Mouse
CD5L 背景信息
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.
全称
CD5 molecule-like
研究领域
- Immunology>>Adaptive Immunity>>B Cell>>B Cell CD Antigen
- Immunology>>Inflammation / Inflammatory Mediator>>Cells Involved in Inflammation>>Monocyte/Macrophage
- Immunology>>Cluster of Differentiation (CD)>>Dendritic Cell CD Antigen>>Other Dendritic Cell CD Antigen
- Immunology>>Innate Immunity>>Monocytes/Macrophages>>Scavenger Receptors
- Cell Biology>>Apoptosis>>Survivin / IAP
- Immunology>>Cluster of Differentiation (CD)>>T Cell CD Antigen
参考文献
- Resnick, D. et al., 1994, Trends Biochem. Sci. 19: 5-8.
- Gebe, J. A. et al., 1997, J. Biol. Chem. 272 (10): 6151–6158.
- Sarrias, M.R. et al., 2004, Crit. Rev. Immunol. 24: 1-37.
- Mukhopadhyay, S. and Gordon, S., 2004, Immunobiology 209: 39-49.
- Sarrias, M. R. et al.,2005, J. Biol. Chem. 280 (42): 35391–35398.
