Anti-B7-1 rabbit polyclonal antibody at 1:500 dilution.
Lane A: 293T Membrane LysateLysates/proteins at 30 ug per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:60 kDa
Observed band size:58 kDa
(We are unsure as to the identity of these extra bands.)