Anti-B7-1 Antibody

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Anti-B7-1 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-B7-1 Antibody
经验证的应用
WB,ELISA,IHC-P
交叉反应
Reacts with: Human
特异性
Human B7-1
免疫原
Recombinant Human CD80 / B7-1 protein (Catalog#10698-H08H)
制备方法
Produced in rabbits immunized with purified, recombinant Human CD80 / B7-1 (rh CD80 / B7-1; Catalog#10698-H08H; NP_005182.1; Met1-Asn242). CD80 / B7-1 specific IgG was purified by Human CD80 / B7-1 affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-B7-1 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:1000
ELISA 1:25000-1:50000
IHC-P 1:2000-1:10000
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-B7-1 Antibody (Rabbit Polyclonal 抗体) 图片

Immunochemical staining of human B7-1 in human lung with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.
Immunochemical staining of human B7-1 in human hodgkin lymphoma with rabbit polyclonal antibody at 1:5000 dilution, formalin-fixed paraffin embedded sections.

Anti-B7-1 rabbit polyclonal antibody at 1:500 dilution.

Lane A: 293T Membrane Lysate

Lysates/proteins at 30 ug per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:60 kDa

Observed band size:58 kDa

(We are unsure as to the identity of these extra bands.)

Anti-B7-1 Antibody: 别称

Anti-B7 Antibody; Anti-B7-1 Antibody; Anti-B7.1 Antibody; Anti-BB1 Antibody; Anti-CD28LG Antibody; Anti-CD28LG1 Antibody; Anti-LAB7 Antibody

B7-1 背景信息

The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD8, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. The B7/CD28/CTLA4 pathway has the ability to both positively and negatively regulate immune responses. CD8 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
全称
CD80 molecule
参考文献
  • Greenfield EA, et al. (1998) CD28/B7 costimulation: a review. Crit Rev Immunol. 18(5): 389-418.
  • Zang X, et al. (2007) The B7 family and cancer therapy: costimulation and coinhibition. Clin Cancer Res. 13(18 Pt 1): 5271-9.
  • Mir MA, et al. (2008) Signaling through CD80: an approach for treating lymphomas. Expert Opin Ther Targets. 12(8): 969-79.
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