SARS-CoV-2 (2019-nCoV) Nucleocapsid ORF mammalian expression plasmid (Codon Optimized)

COVID-19 Nucleocapsid Research.
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SARS-CoV-2 (2019-nCoV) Nucleocapsid ORF mammalian expression plasmid (Codon Optimized): 产品信息

基因
种属
2019-nCoV
基因长度
1260 bp
序列特征
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system. The translated amino acid sequence is identical with QHD43423.2 except for the point mutations: G335A.
产品特征
Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Nucleoprotein / NP.
质粒
启动子
Enhanced CMV mammalian cell promoter
限制性酶切位点
KpnI + XbaI(6.1kb+1.26kb)
测序引物
T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Coronavirus Nucleocapsid cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SARS-CoV-2 (2019-nCoV) Nucleocapsid ORF mammalian expression plasmid (Codon Optimized): 验证图片

SARS-CoV-2 (2019-nCoV) Nucleocapsid ORF mammalian expression plasmid (Codon Optimized): 别称

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Coronavirus Nucleocapsid 背景信息

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.
参考文献
    1. 1.Van Boheemen S, et al. (2012), MBio. 3(6):e00473-12.
    2. Bisht H. et al., 2004, Proc Natl Acad Sci. 101 (17): 6641-6.
    3. Li W. et al., 2005, Science. 309 (5742): 1864-8.
  • A single dose of recombinant VSV-ΔG-spike vaccine provides protection against SARS-CoV-2 challenge
    Author
    Yahalom-Ronen, Y;Tamir, H;Melamed, S;Politi, B;Shifman, O;Achdout, H;Vitner, E;Israeli, O;Milrot, E;Stein, D;Cohen-Gihon, I;Lazar, S;Gutman, H;Glinert, I;Cherry, L;Vagima, Y;Lazar, S;Weiss, S;Ben-Shmuel, A;Avraham, R;Puni, R;Lupu, E;Bar David, E;Sittner, A;Erez, N;Zichel, R;Mamroud, E;Mazor, O;Levy, H;Laskar, O;Yitzhaki, S;Shapira, S;Zvi, A;Beth-Din, A;Paran, N;Israely, T;
    Year
    2020
    Journal
    bioRxiv
    Application
    template
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