SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-Myc tag (Codon Optimized)

COVID-19 Spike S1+S2 ECD Research.
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SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-Myc tag (Codon Optimized): 产品信息

基因
种属
2019-nCoV
基因长度
3867 bp
序列特征
A number of silent mutations were introduced into the DNA sequence in order to increase its protein expression level in mammalian cell system.The translated amino acid sequence is identical with QHD43416.1
产品特征
Full length Clone DNA of SARS-CoV-2 (2019-nCoV) Spike.
质粒
启动子
Enhanced CMV mammalian cell promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+3.87kb)
标签序列
Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
测序引物
T7(TAATACGACTCACTATAGGG) BGH(TAGAAGGCACAGTCGAGG)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Coronavirus spike cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-Myc tag (Codon Optimized): 验证图片

SARS-CoV-2 (2019-nCoV) Spike Gene ORF cDNA clone expression plasmid, C-Myc tag (Codon Optimized): 别称

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Coronavirus spike 背景信息

The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that SARS-CoV-2 (COVID-19 coronavirus, 2019-nCoV) can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
参考文献
  • Shen S, et al. (2007) Expression, glycosylation, and modification of the spike (S) glycoprotein of SARS CoV. Methods Mol Biol. 379: 127-35.
  • Du L, et al. (2009) The spike protein of SARS-CoV--a target for vaccine and therapeutic development. Nat Rev Microbiol. 7 (3): 226-36.
  • Xiao X, et al. (2004) The SARS-CoV S glycoprotein. Cell Mol Life Sci. 61 (19-20): 2428-30.

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