AMFR cDNA ORF Clone, Human, C-OFPSpark® tag
AMFR cDNA ORF Clone, Human, C-OFPSpark® tag: 产品信息
基因
种属
Human
NCBI 参考序列号
基因长度
2619 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human autocrine motility factor receptor.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6kb+2.62kb)
标签序列
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
AMFR cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
关于 AMFR的更多信息
Entrez Gene ID
AMFR cDNA ORF Clone, Human, C-OFPSpark® tag: 验证图片
AMFR cDNA ORF Clone, Human, C-OFPSpark® tag: 别称
AMFR cDNA ORF Clone, Human
AMFR 背景信息
Autocrine motility factor receptor (AMFR/gp78), a receptor for autocrine motility factor (AMF), is a cell surface glycoprotein of 78000 molecular weight, regulating cell motility signaling in vitro and metastasis in vivo. AMFR, is also localized to the endoplasmic reticulum where it functions as an E3 ubiquitin ligase. Expression of both AMF/PGI and gp78/AMFR is associated with cancer. AMF and AMFR are overexpressed in human breast cancer and are negatively associated with patients' clinical outcome. The AMF-AMFR complex plays an important role in the progression of breast cancer, as well as having a prognostic role.
参考文献
- Onishi Y, et al. (2003) Overexpression of autocrine motility factor receptor (amfr) in nih3t3 fibroblasts induces cell transformation. Clin Exp Metastasis 20 (1): 51-58.
- Jiang WG, et al. (2006) Expression of autocrine motility factor (amf) and its receptor, amfr, in human breast cancer. J Histochem Cytochem 54 (2): 231-241.
- Fairbank M, et al. (2009) The complex biology of autocrine motility factor/phosphoglucose isomerase (amf/pgi) and its receptor, the gp78/amfr e3 ubiquitin ligase. Mol Biosyst 5 (8): 793-801.
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