CA5A cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag

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CA5A cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
957 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human carbonic anhydrase VA, mitochondrial with C terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.96kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

CA5A cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

CA5A cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 验证图片

CA5A cDNA ORF Clone, Human, C-DYKDDDDK (Flag®) tag: 别称

CA5 cDNA ORF Clone, Human; CA5AD cDNA ORF Clone, Human; CA5D cDNA ORF Clone, Human; Carbonic Anhydrase VA cDNA ORF Clone, Human; CAV cDNA ORF Clone, Human; CAVA cDNA ORF Clone, Human; GS1-21A4.1 cDNA ORF Clone, Human

CA5A 背景信息

Carbonic anhydrase 5A, mitochondrial, also known as Carbonate dehydratase VA, Carbonic anhydrase VA, CA-VA and CA5A, is a member of thealpha-carbonic anhydrase family. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA5A / CA-VA is activated by histamine, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. It is inhibited by coumarins, sulfonamide derivatives such as acetazolamide and Foscarnet (phosphonoformate trisodium salt).
全称
carbonic anhydrase VA, mitochondrial
参考文献
  • Strausberg, R.L. et al., 2002, Proc. Natl. Acad. Sci. USA 99:16899 - 903.
  • Liao, S.Y. et al., 2003, J. Med. Genet. 40:257 - 262.
  • Temperini C.et al., 2006, Chemistry 12: 7057-66.
  • Temperini C.et al., 2006, J. Med. Chem. 49: 3019-27.
  • Supuran, C. T. et al., 2008, Curr Pharm Des. 14 (7): 601-602.
  • Elleuche, S. et al., 2009, Curr Genet. 55 (2): 211-222.
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