Carbonic anhydrase II cDNA ORF Clone, Human, N-HA tag

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Carbonic anhydrase II cDNA ORF Clone, Human, N-HA tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
825 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 604C/T not causing the amino acid variation.
产品特征
Full length Clone DNA of Human carbonic anhydrase II with N terminal HA tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.83kb)
标签序列
HA Tag Sequence: TATCCTTACGACGTGCCTGACTACGCC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Carbonic anhydrase II cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Carbonic anhydrase II cDNA ORF Clone, Human, N-HA tag: 验证图片

Carbonic anhydrase II cDNA ORF Clone, Human, N-HA tag: 别称

CA-II cDNA ORF Clone, Human; CAC cDNA ORF Clone, Human; CAII cDNA ORF Clone, Human; Car2 cDNA ORF Clone, Human; HEL-76 cDNA ORF Clone, Human; HEL-S-282 cDNA ORF Clone, Human

Carbonic anhydrase II 背景信息

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase II is one of fourteen forms of human α carbonic anhydrases. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of sodium ions in the proximal tubule. Carbonic anhydrase II has been shown to interact with Band 3 and Sodium-hydrogen antiporter 1.
全称
carbonic anhydrase II
参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Lilias A, et al. (1972) Crystal Structure of Human Carbonic Anhydrase C. Nature new biology. 235: 131-7.
  • Li XJ, et al. (2002) Carbonic Anhydrase II Binds to and Enhances Activity of the Na+/H+ Exchanger. The Journal of Biological Chemistry. 277: 36085-91.
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