Cytokeratin 5/KRT5 cDNA ORF Clone, Human, N-His tag

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Cytokeratin 5/KRT5 cDNA ORF Clone, Human, N-His tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1818 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human keratin 5 with N terminal His tag.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+1.82kb)
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Cytokeratin 5/KRT5 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Cytokeratin 5/KRT5 cDNA ORF Clone, Human, N-His tag: 验证图片

Cytokeratin 5/KRT5 cDNA ORF Clone, Human, N-His tag: 别称

CK5 cDNA ORF Clone, Human; DDD cDNA ORF Clone, Human; DDD1 cDNA ORF Clone, Human; EBS2 cDNA ORF Clone, Human; K5 cDNA ORF Clone, Human; KRT5A cDNA ORF Clone, Human

Cytokeratin 5/KRT5 背景信息

Keratin 5 is the major type II keratin of the basal cells of epidermis and of other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP.
全称
keratin 5, type II
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