MMP13 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag

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MMP13 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1416 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human matrix metallopeptidase 13 (collagenase 3) with N terminal Flag tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.45kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

MMP13 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

MMP13 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat# STF02). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

MMP13 cDNA ORF Clone, Human, N-DYKDDDDK (Flag®) tag: 别称

CLG3 cDNA ORF Clone, Human; MANDP1 cDNA ORF Clone, Human; MMP-13 cDNA ORF Clone, Human

MMP13 背景信息

Matrix metalloproteinases (MMPs) such as MMP13 promote tumor growth and progression by mediating extracellular matrix (ECM) reorganization and regulating the biological activity of cytokines. Matrix metalloproteinase 13 (MMP13) plays a central role in the MMP activation cascade that enables degradation of the extracellular matrix and basement membranes, and it is identified as a potential driver in oral carcinogenesis.MMP13 is enriched in mature chondrocytes and is considered a prime cause of ECM degradation in the osteoarthritic articular cartilage in temporomandibular joints. A missense mutation of MMP13 causes the Missouri type of human Spondyloepimetaphyseal dysplasia (SEMD(MO)), an autosomal dominant disorder characterized by defective growth and modeling of vertebrae and long bones.
全称
matrix metallopeptidase 13
研究领域
Related Pathways
  • IL17 signaling pathway
    IL17 signaling pathway
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