The plasmid is confirmed by full-length sequencing.
Stable or Transient mammalian expression
储存 & 运输
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
Nrf2/NFE2L2 cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
The nuclear factor, erythroid 2 like 2 (NFE2L2) is an important transcription factor that protects cells from oxidative stress (OS). NFE2L2 deficiency in the placentas is associated with pregnancy complications. Phosphorylation of the transcription factor NFE2L2 controls mRNA abundance of genes encoding antioxidant proteins and enzymes. NFE2L2 pathway contributes to the environmental resistance of cancers by enhancing the antioxidant capacity. Nrf2 (encoded in humans by the NFE2L2 gene) is the master regulator of antioxidant defenses, a transcription factor that regulates the expression of more than 200 cytoprotective genes. Increasing evidence indicates that Nrf2 signaling plays a key role in how oxidative stress mediates the beneficial effects of exercise. NRF2 and its principal negative regulator, the E3 ligase adaptor Kelch-like ECH-associated protein 1 (KEAP1), are critical in the maintenance of redox, metabolic and protein homeostasis, as well as the regulation of inflammation.
Wu J, et al. (2017) Placental nfe2l2 is discordantly activated in monochorionic twins with selective intrauterine growth restriction and possibly regulated by hypoxia. Free Radic Res 51 (4): 351-359.
Jung KA, et al. (2017) Nfe2l2/nrf2 activity is linked to mitochondria and amp-activated protein kinase signaling in cancers through mir-181c/mitochondria-encoded cytochrome c oxidase regulation. Antioxid Redox Signal 27 (13): 945-961.
Han LQ, et al. (2018) Phosphorylation of nuclear factor erythroid 2-like 2 (nfe2l2) in mammary tissue of holstein cows during the periparturient period is associated with mrna abundance of antioxidant gene networks. J Dairy Sci 101 (7): 6511-6522.
Done AJ, et al. (2016) Nrf2 mediates redox adaptations to exercise. Redox Biol 10 191-199.