p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-GFPSpark® tag

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p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-GFPSpark® tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1224 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human cyclin-dependent kinase inhibitor 1A (p21, Cip1) with C terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6kb+1.22kb)
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

p21/WAF1/CDKN1A cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-GFPSpark® tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

p21/WAF1/CDKN1A cDNA ORF Clone, Human, C-GFPSpark® tag: 别称

CAP20 cDNA ORF Clone, Human; CDKN1 cDNA ORF Clone, Human; CIP1 cDNA ORF Clone, Human; MDA-6 cDNA ORF Clone, Human; P21 cDNA ORF Clone, Human; p21CIP1 cDNA ORF Clone, Human; SDI1 cDNA ORF Clone, Human; WAF1 cDNA ORF Clone, Human

p21/WAF1/CDKN1A 背景信息

全称
cyclin-dependent kinase inhibitor 1A (p21, Cip1)
研究领域
Related Pathways
  • p53 Pathway
    p53 Pathway
  • AKT Signaling Pathway
    AKT Signaling Pathway
  • AMPK Signaling Pathway
    AMPK Signaling Pathway
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