PI3K gamma/Pik3cg cDNA ORF Clone, Human, untagged

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PI3K gamma/Pik3cg cDNA ORF Clone, Human, untagged: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
3309 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 972G/A,981C/T,2025T/C not causing the amino acid variation.
产品特征
Full length Clone DNA of Human phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit gamma.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6.1kb+3.31kb)
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

PI3K gamma/Pik3cg cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

PI3K gamma/Pik3cg cDNA ORF Clone, Human, untagged: 验证图片

PI3K gamma/Pik3cg 背景信息

Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I catalytic subunit of PI3K. Like other class I catalytic subunits (p11-alpha p11-beta, and p11-delta), the encoded protein binds a p85 regulatory subunit to form PI3K. This gene is located in a commonly deleted segment of chromosome 7 previously identified in myeloid leukemias. Several transcript variants encoding the same protein have been found for this gene.
Related Pathways
  • Common G-Chain signaling pathway
    Common G-Chain signaling pathway
参考文献
  • Kächele M, Hennige AM, Machann J, et al. Variation in the Phosphoinositide 3-Kinase Gamma Gene Affects Plasma HDL-Cholesterol without Modification of Metabolic or Inflammatory Markers. Zhang Y, ed. PLoS ONE. 2015;10(12):e0144494.
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