Sphingosine Kinase 1 cDNA ORF Clone, Human, N-His tag

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Sphingosine Kinase 1 cDNA ORF Clone, Human, N-His tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
1200 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1008T/C not causing the amino acid variation.
产品特征
Full length Clone DNA of Human sphingosine kinase 1.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+1.2kb)
标签序列
His Tag Sequence: CACCATCACCACCATCATCACCACCATCAC
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Sphingosine Kinase 1 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

Sphingosine Kinase 1 cDNA ORF Clone, Human, N-His tag: 验证图片

Sphingosine Kinase 1 cDNA ORF Clone, Human, N-His tag: 别称

SPHK cDNA ORF Clone, Human; SPHK1 cDNA ORF Clone, Human; SPK cDNA ORF Clone, Human

Sphingosine Kinase 1 背景信息

SPHK1, also known as sphingosine Kinase 1, catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P). S1P exhibits a broad spectrum of biological activities including cell proliferation, survival, migration, cytoskeletal organization, and morphogenesis. It is a ligand for cell surface G protein-coupled receptors. SPHK 1 is a potential therapeutic target for the control of cancer and inflammation. SPHK1 plays a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes.
全称
sphingosine kinase 1
参考文献
  • Kohama T. et al., 1998, J Biol Chem. 273 (37): 23722-8.
  • Xia P. et al., 2002, J Biol Chem. 277 (10): 7996-8003.
  • Tsukahara T. et al., 2002, Tanpakushitsu Kakusan Koso. 47 (4): 509-13.
  • N-AS-triggered SPMs are direct regulators of microglia in a model of Alzheimer's disease
    Author
    Lee, JY;Han, SH;Park, MH;Song, IS;Choi, MK;Yu, E;Park, CM;Kim, HJ;Kim, SH;Schuchman, EH;Jin, HK;Bae, JS;
    Year
    2020
    Journal
    Nat Commun
    Application
    expression
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