The plasmid is confirmed by full-length sequencing.
储存 & 运输
Each tube contains lyophilized plasmid.
The lyophilized plasmid can be stored at ambient temperature for three months.
SUCLA2 cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
SUCLA2 encodes the ATP-forming β subunit (A-SUCL-β) of succinyl-CoA ligase, an enzyme of the citric acid cycle. Sucla2 is located in the mitochondrial matrix and catalyzes the reversible synthesis of succinate and adenosine triphosphate (ATP) in the tricarboxylic acid cycle. Mutations in SUCLA2, encoding the ß-subunit of succinyl-CoA synthetase of the Krebs cycle, are one cause of mitochondrial DNA depletion syndrome. Mutations in SUCLA2 lead to a mitochondrial disorder manifesting as encephalomyopathy with dystonia, deafness, and lesions in the basal ganglia. Patients have been reported to have severe progressive childhood-onset encephalomyopathy, and methylmalonic aciduria, often leading to death in childhood. Sucla2 expression was found to be correlated with the capacitation of boar spermatozoa. The metabolic vulnerability in SUCLA2-deficient prostate cancer cells is pharmacologically targetable.
succinate-CoA ligase, ADP-forming, beta subunit
Matilainen S, et al. (2017) Mitochondrial encephalomyopathy and retinoblastoma explained by compound heterozygosity of sucla2 point mutation and 13q14 deletion. Eur J Hum Genet 25 (3): 393.
Huang S, et al. (2016) Knockdown of sucla2 decreases the viability of mouse spermatocytes by inducing apoptosis through injury of the mitochondrial function of cells. Folia Histochem Cytobiol 54 (3): 134-142.
Dobolyi A, et al. (2015) Exclusive neuronal expression of sucla2 in the human brain. Brain Struct Funct 220 (1): 135-151.