TCF4/E2-2 cDNA ORF Clone, Human, C-GFPSpark® tag

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TCF4/E2-2 cDNA ORF Clone, Human, C-GFPSpark® tag: 产品信息

基因
种属
Human
NCBI 参考序列号
基因长度
2745 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 1941A/G not causing the amino acid variation.
产品特征
Full length Clone DNA of Human transcription factor 4 with C terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + NotI(6kb+2.75kb)
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

TCF4/E2-2 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

TCF4/E2-2 cDNA ORF Clone, Human, C-GFPSpark® tag: 验证图片

TCF4/E2-2 cDNA ORF Clone, Human, C-GFPSpark® tag: 别称

bHLHb19 cDNA ORF Clone, Human; E2-2 cDNA ORF Clone, Human; ITF-2 cDNA ORF Clone, Human; ITF2 cDNA ORF Clone, Human; PTHS cDNA ORF Clone, Human; SEF-2 cDNA ORF Clone, Human; SEF2 cDNA ORF Clone, Human; SEF2-1 cDNA ORF Clone, Human; SEF2-1A cDNA ORF Clone, Human; SEF2-1B cDNA ORF Clone, Human; SEF2-1D cDNA ORF Clone, Human; TCF-4 cDNA ORF Clone, Human

TCF4/E2-2 背景信息

Transcription factor 7 like 2 (TCF7L2, also known as TCF4) is a Wnt signaling pathway transcription factor involved in the regulation of numerous Wnt targeted genes. TCF4 is critical in the vertebrate intestinal epithelium, where TCF4-β-catenin complexes are necessary for the maintenance of a proliferative compartment, and their abnormal formation initiates tumorigenesis. β-catenin, an upstream protein of TCF4, accumulates in the cytoplasm, then translocates to the nucleus to activate the β-catenin/T-cell factor/lymphoid enhancer factor (TCF/LEF) transcriptional machinery and regulates target genes. Previous studies showed that TCF4 was involved in cell proliferation and apoptosis. Human haploinsufficiency of the transcription factor Tcf4 leads to a rare autism spectrum disorder called Pitt-Hopkins syndrome (PTHS), which is associated with severe language impairment and development delay.
全称
transcription factor 4
Related Pathways
  • Canonical Wnt Pathway
    Canonical Wnt Pathway
  • Non-Canonical Wnt Pathway
    Non-Canonical Wnt Pathway
参考文献
  • Liu X, et al. (2016) T-cell factor (tcf/lef1) binding elements (tbes) of fasl (fas ligand or cd95 ligand) bind and cluster fas (cd95) and form complexes with the tcf-4 and b-catenin transcription factors in vitro and in vivo which result in triggering cell death and/or cell activation. Cell Mol Neurobiol 36 (6): 1001-1013.
  • Lan F, et al. (2012) Genome-wide identification of tcf7l2/tcf4 target mirnas reveals a role for mir-21 in wnt-driven epithelial cancer. Int J Oncol 40 (2): 519-526.
  • Kennedy AJ, et al. (2016) Tcf4 regulates synaptic plasticity, DNA methylation, and memory function. Cell Rep 16 (10): 2666-2685.
  • Costa AM, et al. (2013) Grg5/aes interacts with t-cell factor 4 (tcf4) and downregulates wnt signaling in human cells and zebrafish embryos. PLoS One 8 (7): e67694.
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