Vimentin cDNA ORF Clone, Human, N-OFPSpark® tag
Vimentin cDNA ORF Clone, Human, N-OFPSpark® tag: 产品信息
基因
种属
Human
NCBI 参考序列号
基因长度
2088 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Human vimentin.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI(6kb+2.09kb)
标签序列
OFPSpark Tag: GATAGCACTGAG……CACCTGTTCCAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Vimentin cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
Vimentin cDNA ORF Clone, Human, N-OFPSpark® tag: 验证图片
Vimentin cDNA ORF Clone, Human, N-OFPSpark® tag: 别称
CTRCT30 cDNA ORF Clone, Human; HEL113 cDNA ORF Clone, Human
Vimentin 背景信息
Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.
全称
vimentin
参考文献
- Russell RL, et al. (2001) Uridine phosphorylase association with vimentin. Intracellular distribution and localization. J Biol Chem. 276(16):13302-7.
- Moinova, et al. (2012) Aberrant Vimentin Methylation is Characteristic of Upper GI Pathologies. Cancer Epidemiology Biomarkers Prev. 21(4):594-600.
- Leader M, et al. (1987) Vimentin: an evaluation of its role as a tumour marker. Histopathology. 11(1):63-72.
Vimentin cDNA ORF Clone, Human, 相关产品
Vimentin cDNA ORF Clone, Mouse, 相关产品
Vimentin cDNA ORF Clone, Rat, 相关产品
If you couldn't find the specific citation, you can:
