Argonaute-2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag

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Argonaute-2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
2583 bp
序列特征
Identical with the Gene Bank Ref. ID sequence (Nucleotide may contain silent mutation without changing amino acid sequence)
产品特征
Full length Clone DNA of Mus musculus eukaryotic translation initiation factor 2C, 2.
质粒
启动子
Enhanced CMV mammalian cell promoter
标签序列
GFPSpark Tag Sequence: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
pLen-F(CTCGTTTAGTGAACCGTCAGAATT), pLen-R(GAACCGGAACCCTTAAACATGT)
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Ampicillin
储存 & 运输
运输方式
Each tube contains 10μg lyophilized plasmid
储存条件
The lyophilized plasmid can be stored at room temperature for three months

关于 Argonaute-2的更多信息

Entrez Gene ID

Argonaute-2 Lentiviral cDNA ORF Clone, Mouse, C-GFPSpark® tag: 别称

1110029L17Rik cDNA ORF Clone, Mouse; 2310051F07Rik cDNA ORF Clone, Mouse; Ago2 cDNA ORF Clone, Mouse; AI225898 cDNA ORF Clone, Mouse; AL022874 cDNA ORF Clone, Mouse; AW546247 cDNA ORF Clone, Mouse; Eif2c2 cDNA ORF Clone, Mouse; ENSMUSG00000072493 cDNA ORF Clone, Mouse; Gerp95 cDNA ORF Clone, Mouse; Gm10365 cDNA ORF Clone, Mouse; KIAA4215 cDNA ORF Clone, Mouse; mKIAA4215 cDNA ORF Clone, Mouse

Argonaute-2 背景信息

Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
全称
argonaute RISC catalytic component 2
参考文献
  • O'Carroll D, et al. (2007) A Slicer-independent role for Argonaute 2 in hematopoiesis and the microRNA pathway. Genes Dev. 21(16): 1999-2004.
  • Pepper AS, et al. (2009) Argonaute2 suppresses Drosophila fragile X expression preventing neurogenesis and oogenesis defects. PLoS One. 4(10): e7618.
  • Lima WF, et al. (2009) Binding and cleavage specificities of human Argonaute2. J Biol Chem. 284(38): 26017-28.
  • Adams BD, et al. (2009) Argonaute-2 expression is regulated by epidermal growth factor receptor and mitogen-activated protein kinase signaling and correlates with a transformed phenotype in breast cancer cells. Endocrinology. 150(1): 14-23.
  • Salvatore V, et al. (2010) Bacterial expression of mouse argonaute 2 for functional and mutational studies. Int J Mol Sci. 11(2): 745-53.
  • Wilson JA, et al. (2011) Human Ago2 is required for efficient microRNA 122 regulation of hepatitis C virus RNA accumulation and translation. J Virol. 85(5): 2342-50.
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