B7-H4 cDNA ORF Clone, Mouse, C-GFPSpark® tag

1/2
Price:
Size:
Number:

B7-H4 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
852 bp
序列特征
Identical with the Gene Bank Ref. ID sequence except for the point mutations: 570T>C, 585C>G, 687C>T, 705C>T, 744A>G, 757T>C, 762G>C, 768T>G and 816A>G not causing the amino acid variation.
产品特征
Full length Clone DNA of Mouse V-set domain containing T cell activation inhibitor 1 with C terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
限制性酶切位点
KpnI + XbaI (6kb + 1.58kb)
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

B7-H4 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

B7-H4 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 验证图片

The plasmid was transfected into 293H adherent cells with Sinofection reagent (Cat#STF01) transiently. After 48 h, the fluorescent signals can be detected by fluorescence microscope. Green excitation 475/40nm, emission 535/45nm. Red excitation 560/55nm, emission 645/45nm. Orange excitation 525/45nm, emission 595/60nm.

B7-H4 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 别称

B7h4 cDNA ORF Clone, Mouse; B7s1 cDNA ORF Clone, Mouse; B7x cDNA ORF Clone, Mouse; BC032925 cDNA ORF Clone, Mouse

B7-H4 背景信息

V-set domain-containing T-cell activation inhibitor 1, also known as B7X, B7H4, B7S1, and VTCN1, is a single-pass type-III membrane protein belonging to the B7 family of costimulatory proteins. These proteins are expressed on the surface of antigen-presenting cells and interact with ligands on T lymphocytes. They provide costimulatory signals that regulate T cell responses. A soluble form of B7H4 has also been detected. B7X / VTCN1 / B7H4 negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, B7X / VTCN1 / B7H4 plays an important role, together with regulatory T-cells(Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. B7X / VTCN1 / B7H4 is also involved in promoting epithelial cell transformation. This membrane protein can be up-regulated by IL6 / interleukin-6 and IL10 / interleukin-10 and inhibited by CSF2 / GM-CSF and IL4 / interleukin-4 on antigen-presenting cells.
全称
V-set domain containing T cell activation inhibitor 1
研究领域
参考文献
  • Zang X, et al. (2003) B7x: a widely expressed B7 family member that inhibits T cell activation. Proc Natl Acad Sci U S A. 100(18): 10388-92.
  • Suh WK, et al. (2006) Generation and characterization of B7-H4/B7S1/B7x-deficient mice. Mol Cell Biol. 26(17): 6403-11.
  • Zang X, et al. (2007) B7-H3 and B7x are highly expressed in human prostate cancer and associated with disease spread and poor outcome. Proc Natl Acad Sci U S A. 104(49):19458-63.

B7-H4 cDNA ORF Clone, Rat, 相关产品

B7-H4 cDNA ORF Clone, Rhesus, 相关产品

添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.