IFI30 cDNA ORF Clone, Mouse, C-GFPSpark® tag

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IFI30 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
747 bp
产品特征
Full length Clone DNA of Mouse interferon gamma inducible protein 30 with C terminal GFPSpark tag.
质粒
启动子
Enhanced CMV promoter
标签序列
GFPSpark: GTGAGCAAGGGC……GAGCTGTACAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' )
BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.

Citation List

IFI30 cDNA ORF Clone, Mouse, C-GFPSpark® tag: 别称

GILT cDNA ORF Clone, Mouse; IP30 cDNA ORF Clone, Mouse

IFI30 背景信息

IFI3 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL8. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI3 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI3 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.
全称
interferon gamma inducible protein 30
参考文献
  • Haque MA, et al. (2002) Absence of gamma-Interferon-inducible Lysosomal Thiol Reductase in Melanomas Disrupts T Cell Recognition of Select Immunodominant Epitopes. J Exp Med. 195(10):1267-77.
  • Phan UT, et al. (2000) Gamma-interferon-inducible lysosomal thiol reductase (GILT). Maturation, activity, and mechanism of action. J Biol Chem. 275(34):25907-14.
  • Phan UT, et al. (2001) Multiple species express thiol oxidoreductases related to GILT. Immunogenetics. 53(4):342-6.
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