ZDHHC12 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag
ZDHHC12 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 产品信息
基因
种属
Mouse
NCBI 参考序列号
基因长度
843 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Mouse zinc finger, DHHC domain containing 12.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.84kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.
ZDHHC12 cDNA ORF 核苷酸序列及氨基酸序列信息
**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**
关于 ZDHHC12的更多信息
Entrez Gene ID
ZDHHC12 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 验证图片
The plasmid was transfected into 293E adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.
ZDHHC12 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 别称
1190004A01Rik cDNA ORF Clone, Mouse
ZDHHC12 背景信息
ZDHHC12 (Zinc Finger DHHC-Type Palmitoyltransferase 12) is a Protein Coding gene. The ZDHHC12 gene, located on 9q34.11, is conserved in chimpanzee, dog, cow, mouse, rat, chicken, zebrafish, and frog. ZDHHC12 belongs to the DHHC palmitoyltransferase family. 3 alternatively spliced human isoforms have been reported. ZDHHC12 is widely expressed in the colon, stomach, and other tissues. ZDHHC12-mediated S-palmitoylation promotes the cell membrane localization of claudin-3 (CLDN3) and maintains its stability in ovarian cancer cells, contributing to the oncoprotein function of CLDN3. Targeting ZDHHC12-mediated CLDN3 S-palmitoylation might be a potential strategy for ovarian cancer therapy. 225 organisms have orthologs with the human gene ZDHHC12.
全称
zinc finger, DHHC-type containing 12
参考文献
- Yuan M, et al. (2020) Zdhhc12-mediated claudin-3 s-palmitoylation determines ovarian cancer progression. Acta Pharm Sin B 10 (8): 1426-1439.
- Ohno Y, et al. (2006) Intracellular localization and tissue-specific distribution of human and yeast dhhc cysteine-rich domain-containing proteins. Biochim Biophys Acta 1761 (4): 474-483.
- Dejanovic B, et al. (2014) Palmitoylation of gephyrin controls receptor clustering and plasticity of gabaergic synapses. PLoS Biol 12 (7): e1001908.
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