ZDHHC3 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag

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ZDHHC3 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 产品信息

基因
种属
Mouse
NCBI 参考序列号
基因长度
939 bp
序列特征
Identical with the Gene Bank Ref. ID sequence.
产品特征
Full length Clone DNA of Mouse zinc finger, DHHC domain containing 3.
质粒
启动子
Enhanced CMV promoter
载体
限制性酶切位点
KpnI + XbaI(6kb+0.94kb)
标签序列
FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
测序引物
T7( 5' TAATACGACTCACTATAGGG 3' ) BGH( 5' TAGAAGGCACAGTCGAGG 3' )
质控
The plasmid is confirmed by full-length sequencing.
筛选
细菌筛选抗性
Kanamycin
细胞筛选抗性
Hygromycin
应用
Stable or Transient mammalian expression
储存 & 运输
运输方式
Each tube contains lyophilized plasmid.
储存条件
The lyophilized plasmid can be stored at ambient temperature for three months.
Note: Flag® is a registered trademark of Sigma Aldrich Biotechnology LP. It is used here for informational purposes only.

ZDHHC3 cDNA ORF 核苷酸序列及氨基酸序列信息

**Sino Biological guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories.**

关于 ZDHHC3的更多信息

Entrez Gene ID

ZDHHC3 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 验证图片

The plasmid was transfected into 293E adherent cells with Sinofection reagent (Cat# STF01). After 48 h, Immunofluorescence staining of cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Flag Tag monoclonal antibody (CST#8146S) at 37℃ 1 hour. Then cells were stained with Goat Anti-mouse IgG secondary antibody. The fluorescent signal is detected by fluorescence microscope. Each expression experiment has negative control.

ZDHHC3 cDNA ORF Clone, Mouse, C-DYKDDDDK (Flag®) tag: 别称

1110020O22Rik cDNA ORF Clone, Mouse; 1810006O10Rik cDNA ORF Clone, Mouse; 2210017C02Rik cDNA ORF Clone, Mouse; DHHC-3 cDNA ORF Clone, Mouse; GODZ cDNA ORF Clone, Mouse; Gramp1 cDNA ORF Clone, Mouse; ZDHHC3 cDNA ORF Clone, Mouse; Zfp373 cDNA ORF Clone, Mouse

ZDHHC3 背景信息

ZDHHC3, also known as GODZ (Golgi apparatus-specific DHHC zinc finger protein), has been shown to palmitoylate AMPA, NMDA, and GABAA receptors and hence to control their trafficking at the synapses. Besides, ZDHHC3 can palmitoylate a number of proteins involved in control of neuronal morphogenesis, such as the α6 integrin subunit, Gαs and Gαq proteins, and stathmins 2 and 3. ZDHHC3 utilizes a “ping-pong” kinetic mechanism to transfer palmitate to the target protein, while no regulatory sites on the ZDHHC3 protein have yet been discovered. ZDHHC3 tyrosine residues are specific targets for FGFR1 and Src kinases. DHHC3 is upregulated in malignant and metastatic human breast cancer. Elevated expression of DHHC3 correlated with diminished patient survival in breast cancer and other human cancer types.
全称
zinc finger DHHC-type containing 3
参考文献
  • Sharma C, et al. (2017) Protein acyltransferase dhhc3 regulates breast tumor growth, oxidative stress, and senescence. Cancer Res 77 (24): 6880-6890.
  • Lievens PM, et al. (2016) Zdhhc3 tyrosine phosphorylation regulates neural cell adhesion molecule palmitoylation. Mol Cell Biol 36 (17): 2208-2225.
  • Kinney N, et al. (2017) Zdhhc3 as a risk and mortality marker for breast cancer in african american women. Cancer Inform 16 1176935117746644.
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