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In CHO (Chinese Hamster Ovary) cell transient transfection, large quantities of DNA plasmids containing the target gene of interest are transfected into the host CHO cells, with an efficient transfection reagent. Regular CHO cells have very low transfection efficiency. Sino Biological has developed an engineered CHO cell line. The optimized CHO cells have an improved transfection efficiency and can maintain a certain number of DNA copies within the cells for a short period of time, up to 6-12 days. The advantage of transient transfection is that it totally skipped the need for lengthy and costly stable cell line development, and hence it is highly preferable for quick and high-throughput small scale protein and antibody production.
Sino Biological has also optimized the serum-free culture medium for optimal transfection efficiency and hence improved protein expression yield. Furthermore, Sino Biological has developed a highly efficient, room temperature stable, and cost-effective mammalian cell transfection reagent, Sinofection, that allows very efficient gene transfection, making it possible to produce large quantities of bulk proteins and bulk antibodies efficiently and at affordable costs. Multiple top multi-national pharmaceutical and biotech companies have taken advantages of our cutting-edge transient transfection platform to support their internal antibody discovery and development projects. Starting in 2015, Sino Biological is offering this service to the life science community and biotech industry to accelerate basic research and antibody product discovery and development.
How to Submit a Request for Transient Transfection Service?
f you are interested in using our transient transfection services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROfirstname.lastname@example.org. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.