CRISPR Protocol

Here are some specific experimental methods for the CRISPR Protocol. If necessary, you can refer to the corresponding articles.

1. Genome engineering using the CRISPR-Cas9 system. 2013

2. Improved vectors and genome-wide libraries for CRISPR screening. 2014

3. CRISPR RNA–guided activation of endogenous human genes. 2013

4. Generation of Endogenous BMP Transcriptional Reporter Cells Through CRISPR/Cas9 Genome Editing. 2018

5. Annotation and Classification of CRISPR-Cas Systems. 2018

6. A Simple Protocol for Loss-of-Function Analysis in Xenopus tropicalis Founders Using the CRISPR-Cas System. 2018

7. Creating Knockin Alleles in Mouse Embryonic Stem Cells by CRISPR/Cas9-Mediated Homologous Recombination Without Drug Selection. 2018

8. Production of Genetically Engineered Porcine Embryos by Handmade Cloning. 2018

9. Functional Studies of Transcriptional Cofactors via Microinjection-Mediated Gene Editing in Xenopus. 2018

10. CRISPR/Cas9-Based Gene Dropout Screens. 2018

11. Microinjection of Marine Fish Eggs. 2018

12. A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair. 2018

13. Generation of CRISPR-Edited Rodents Using a Piezo-Driven Zygote Injection Technique. 2018

14. Microinjection and Oviduct Transfer Procedures for Rat Model Generation with CRISPR-Cas9 Technology. 2018

15. Editing the Epigenome: Overview, Open Questions, and Directions of Future Development. 2018

16. Allele-Specific Epigenome Editing. 2018