Anti-Cytokeratin 5 / KRT5 Antibody

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Anti-Cytokeratin 5 / KRT5 Antibody (Rabbit Polyclonal 抗体) 产品信息

产品名称
Anti-Cytokeratin 5 / KRT5 Antibody
经验证的应用
WB,ICC/IF,IP
交叉反应
Reacts with: Human
特异性
Human Cytokeratin 5 / KRT5
免疫原
A synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5
制备方法
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Cytokeratin 5 / KRT5, and purified by antigen affinity chromatography.
来源
Polyclonal Rabbit IgG
纯化
Protein A & Antigen Affinity
缓冲液
0.2 μm filtered solution in PBS
偶联物
Unconjugated
状态
Liquid
运输方式
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
储存条件
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.

Anti-Cytokeratin 5 / KRT5 Antibody (Rabbit Polyclonal 抗体) 经验证的应用

应用 推荐稀释比/用量
WB 1:500-1:2000
ICC-IF 1:300-1:10000
IP 1-4 μL/mg of lysate
Please Note: Optimal concentrations/dilutions should be determined by the end user.

Anti-Cytokeratin 5 / KRT5 Antibody (Rabbit Polyclonal 抗体) 图片

Immunofluorescence staining of KRT5 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human KRT5 polyclonal antibody (1:1000) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.

PDIA4 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

Lane B:0.5 mg MCF-7 Whole Cell Lysate

2 µL anti-PDIA4 rabbit monoclonal antibody and 60 μg of Immunomagnetic beads Protein G.

Primary antibody:

Anti-PDIA4 rabbit monoclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 73 kDa

Observed band size: 73 kDa

Anti-KRT5 rabbit polyclonal antibody at 1:500 dilution

Lane A: MCF7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:62 kDa

Observed band size:55 kDa

(We are unsure as to the identity of these extra bands.)

Anti-Cytokeratin 5 / KRT5 Antibody: 别称

Anti-CK5 Antibody; Anti-DDD Antibody; Anti-DDD1 Antibody; Anti-EBS2 Antibody; Anti-K5 Antibody; Anti-KRT5A Antibody

Cytokeratin 5 / KRT5 背景信息

Keratin 5 is the major type II keratin of the basal cells of epidermis and of other stratified epithelia. With its type I partner, keratin 14, it constitutes a major fraction of the cytoskeleton of the basal cells. Epidermolysis bullosa simplex with mottled pigmentation (EBS-MP) is a rare dermatologic disorder of autosomal dominant inheritance with intraepidermal blistering after minor trauma, reticular hyperpigmentation unrelated to the blistering, nail dystrophy, and mild palmoplantar keratosis. Keratin 5 and keratin 14 are known to be essential for the basal keratinocyte cytoskeleton and are defective in several forms of epidermolysis bullosa simplex. Recently, a 71C-->T transition in the keratin 5 gene (KRT5) causing a P24L substitution was identified in some patients with EBS-MP.
全称
keratin 5, type II
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