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Transient transfection technology is an effective alternative to mouse hybridoma cell culture for mouse antibody production, especially for hybridoma cell lines with low productivity, unstable productivity, mixed clone, etc. By cloning out the antibody VL and VH genes from the original hybridoma cells, one gains the flexibility in producing the antibody by transient transfection, stable CHO cell expression, changing the constant region to different sub-type or even as a chimeric antibody. Once the antibody gene is obtained, the antibody can be re-produced even if one losses the hybridoma cell bank or cell line.
Sino Biological has extensive experience and in-house expertise in mouse antibody gene cloning, chimeric antibody construction, antibody humanization, transient transfection or stable expression of the full mouse antibody. We have built one of the largest capacity for high-throughput small scale antibody production or large scale antibody production. For more information, please refer to our antibody production service.
How to Submit a Request for Antibody Production Service?
If you are interested in using our antibody production services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROemail@example.com. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.