• Cutting-edge technologies
• Most reliable partner
• Unmatched experience:>10,000 Mabs,>6000 Proteins
• Superior quality of products & services
• Unmatched speed of production
• Unmatched cost-saving
Poly-histidine tag (his-tag) has been widely used in recombinant protein expression and production. The his-tag allows simple and efficient purification of the tagged protein using Immobilized metal (Nickel or Copper) ion affinity chromatography (IMAC). The his-tag also can be used for detection in western blot by using a his-tag-specific antibody. His-tag can be added to either C- or N-terminus of a protein for expression in virtually all expression systems.
In the past couple of years, Sino Biological has successfully produced many proteins with a his-tag and purified with IMAC columns. We are highly experienced in designing the his-tag according to the protein structure and handle any problems arising from protein purification process. Sino Biological has also developed highly sensitive His-tag specific antibodies for detection in western blot and ELISA assays. For more information on our his-tag epitope antibody, please refer to: Tag antibody / antibodies.
Our track-records in the past seven years (2008-2014) on the successful production of over 5000 recombinant protein bulks and over 10,000 monoclonal antibodies have attracted top-10 multi-national pharmaceutical customers as well as small biotech companies worldwide through word-of-mouth, as we have not done much business development or advertisement in the past. In 2015, we are starting to promote our protein production services to all research institutions and companies worldwide, as we believe more customers can benefit greatly from our capacity and experience.
How to Submit a Request for Protein Production Service?
If you are interested in using our protein production services to support your antibody discovery and development program, please contact us by leaving an on-line message or send us your detailed request to CROfirstname.lastname@example.org. Based on the quantity and quality requirement of your request, a formal quote will be send back to you for your review, comment, and/or acceptance.