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E. coli. is most widely employed in protein expression and production, especially for small proteins. It is easy to master the technique and easy to implement in virtually any biology laboratory. There are many choices in terms of expression vectors and host cell strains. In many cases, protein in E. coli. system is expressed as insolvable inclusion body, which makes it easy to separate the over-expressed protein from other host cell proteins. However, one would then need to solubilize the inclusion body, followed by refolding into correct protein structure and restore its biological function.
With over 10,000 cDNA clone genes as catalog products, Sino Biological offers value added expression-ready vectors and customized vector construction service to scientists worldwide. One can choose various detection and purification tags, such as FLAG, HIS, Myc, HA, GST, etc. Our professional staff are well trained and have many years of hands-on molecule biology experience. Our microbial expression vector construction service is reliable, cost-effective, and fast.
How to Request for E. coli. Expression Vector Construction Service?
If you are interested in using our E. coli. expression vector construction service, please contact us by leaving an on-line message, filling out an on-line inquiry form, or sending us your detailed requests to CROfirstname.lastname@example.org. A formal quote will be send back to you for your review, comment, and/or acceptance.