E. Coli. Expression Vector Construction Service: spET-C-His Vector

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E. Coli. Expression Vector Construction Service: spET-C-His Vector

Service: Construction of protein expression vector E. Coli. expression
Required Material : Target protein gene provided by customer or ordered from Sino Biological
Vector Backbone: spET vector with C-terminus His-Tag
DetectionTag: Poly-histidine tag (His-tag, 6X His tag to 10X His tag)
Purification Tag: His-tag through Immobilized metal ion affinity chromatography (IMAC)
Deliverable: 10ug purified E. Coli. expression vector plasmid
Timeline: 1-2 weeks
Price Quote:  

spET-C-his E. Coli. Expression Vector Physical Map

spET-C-His E. Coli. Expression Vector Description

Schematic of cloning sites on spET-C-His E. Coli. Expression Vector

spET-C-His E. Coli. Expression Vector Backbone DNA Sequence

1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
121 TGTTTAGCAG CCTAGGTATT AATCAATTAC TCGAGTTAGT GGTGATGGTG GTGATGGTGG
181 TGATGGTGTG CGGCCGCAAG CTTGTCGACG GAGCTCGCCT GCAGGCGCGC CAAGGCCTGT
241 ACAGAATTCG GATCCCCGAT ATCCCATGGA CTAGTCATAT GTATATCTCC TTCTTAAAGT
301 TAAACAAAAT TATTTCTAGA GGGGAATTGT TATCCGCTCA CAATTCCCCT ATAGTGAGTC
361 GTATTAATTT CGCGGGATCG AGATCGATCT CGATCCTCTA CGCCGGACGC ATCGTGGCCG
421 GCATCACCGG CGCCACAGGT GCGGTTGCTG GCGCCTATAT CGCCGACATC ACCGATGGGG
481 AAGATCGGGC TCGCCACTTC GGGCTCATGA GCGCTTGTTT CGGCGTGGGT ATGGTGGCAG
541 GCCCCGTGGC CGGGGGACTG TTGGGCGCCA TCTCCTTGCA TGCACCATTC CTTGCGGCGG
601 CGGTGCTCAA CGGCCTCAAC CTACTACTGG GCTGCTTCCT AATGCAGGAG TCGCATAAGG
661 GAGAGCGTCG AGATCCCGGA CACCATCGAA TGGCGCAAAA CCTTTCGCGG TATGGCATGA
721 TAGCGCCCGG AAGAGAGTCA ATTCAGGGTG GTGAATGTGA AACCAGTAAC GTTATACGAT
781 GTCGCAGAGT ATGCCGGTGT CTCTTATCAG ACCGTTTCCC GCGTGGTGAA CCAGGCCAGC
841 CACGTTTCTG CGAAAACGCG GGAAAAAGTG GAAGCGGCGA TGGCGGAGCT GAATTACATT
901 CCCAACCGCG TGGCACAACA ACTGGCGGGC AAACAGTCGT TGCTGATTGG CGTTGCCACC
961 TCCAGTCTGG CCCTGCACGC GCCGTCGCAA ATTGTCGCGG CGATTAAATC TCGCGCCGAT

....... <Complete List of DNA Sequence>

E. Coli. Expression Vector Construction Services

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E. Coli. Expression Vector Construction Service: spET-N-His-NusA Vector
E. Coli. Expression Vector Construction Service: spET-N-His-Sumo Vector
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