E. Coli. Expression Vector Construction Service: spET-N-DsbA-His-EK Vector

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E. Coli. Expression Vector Construction Service: spET-N-DsbA-His-EK Vector

Service: Construction of protein expression vector E. Coli. expression
Required Material : Target protein gene provided by customer or ordered from Sino Biological
Vector Backbone: spET vector with N-terminus His-Tag
DetectionTag: Poly-histidine tag (His-tag, 6X His tag to 10X His tag)
DsbA fusion protein tag
Purification Tag: His-tag through Immobilized metal ion affinity chromatography (IMAC)
Cleavage Site: Cleavage Protease Enzyme: Enterokinase (EK)
Deliverable: 10ug purified E. Coli. expression vector plasmid
Timeline: 1-2 weeks
Price Quote:  

spET-N-DsbA-His-EK E. Coli. Expression Vector Physical Map

spET-N-DsbA-His-EK E. Coli. Expression Vector Description

Schematic of cloning sites on spET-N-DsbA-His-EK E. Coli. Expression Vector

spET-N-DsbA-His-EK E. Coli. Expression Vector Backbone DNA Sequence

1 ATCCGGATAT AGTTCCTCCT TTCAGCAAAA AACCCCTCAA GACCCGTTTA GAGGCCCCAA
61 GGGGTTATGC TAGTTATTGC TCAGCGGTGG CAGCAGCCAA CTCAGCTTCC TTTCGGGCTT
121 TGTTTAGCAG CCTAGGTATT AATCAATTAC TCGAGTGCGG CCGCAAGCTT GTCGACGGAG
181 CTCGCCTGCA GGCGCGCCAA GGCCTGTACA GAATTCGGAT CCCCGATATC CCATGGGACT
241 CTTGTCGTCG TCATCACCGG AGCCACCACC GGTACCCAGA TCTGGGCTGT CCATGTGCTG
301 GCGTTCGAAT TTAGCAGCAG CGGTTTCTTT CATACCAATT GCAGTACTAC CGCGTGGCAC
361 CAGACCCGCG GAGTGATGGT GATGGTGATG ACCAGAACCA CTAGTTGATC CTTTTTTCTC
421 GCTTAAGTAT TTCACTGTAT CAGCATACTG CTGAACAAAA ACATCCATAT TGCTGGTATC
481 CATACCCTGC GGATTCAGCT GATATTTACC GTTAACAAAC ATCGCCGGAA CGCCACGCAA
541 TTGCACGTCA GCTGCAGCTT TTTCCTGCTG AGCGACCAGA GATTTCACCA CGAAGCTGTT
601 CCACGCCGCG TCGTACTCTT CACCTTTAAT ACCTGCGTTG ATAAATACAT CGCGGATATC
661 AGAAGCAGAA CGAATGGTCT GGGTTTTCTG TACGCCTTCA AACAGCGGAA CAGTCACTTT
721 GTCTTCCACG CCCAGCGCCA TCGCCACAGC CCATGCCTGA GTCAGATCTT TGCCCAGGTC
781 ACCACCCATG AAGTTGACGT GGTATTTAGT CATCTTCACG CCTTCCGGCA GTTTTTTCTT
841 CACATTATCA GAAATATGCA GAACTTCTTC AAACTGATAG CAGTGCGGGC AGAAGAAAGA

....... <Complete List of DNA Sequence>

E. Coli. Expression Vector Construction Services

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E. Coli. Expression Vector Construction Service: spET-N-His-EK Vector
E. Coli. Expression Vector Construction Service: spET-N-His-MBP Vector
E. Coli. Expression Vector Construction Service: spET-N-His-NusA Vector
E. Coli. Expression Vector Construction Service: spET-N-His-Sumo Vector
E. Coli. Expression Vector Construction Service: spET-N-OmpA Vector
E. Coli. Expression Vector Construction Service: spET-N-TrxA-His Vector
E. Coli. Expression Vector Construction Service: spET-N-ZZ Vector
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