acnB ELISA Kit, E. coli

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acnB ELISA Kit, E. coli: 产品信息

产品名称
acnB ELISA Kit, E. coli
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
32.4 pg/mL
线性范围
78.1-5000 pg/mL
特异性
Recognizes both recombinant and natural E. coli acnB
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.00 1.93 3.48
SD 0.04 0.08 0.16
CV(%) 3.8% 4.1% 4.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.08 2.18 3.91
SD 0.05 0.19 0.29
CV(%) 5.0% 8.6% 7.3%
回收率
The recovery of E. coli acnB spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
lysate (n=4)
Average % Recovery
107
Range
97-116%
线性关系
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This acnB ELISA Kit, E. coli is an enzyme-linked immunosorbent assay for the quantitative measurement of E. coli acnB protein in Lysate . It contains recombinant E. coli acnB, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

acnB ELISA Kit, E. coli: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.

acnB ELISA Kit, E. coli: 别称

yacI ELISA Kit, E. coli; yacJ ELISA Kit, E. coli

acnB 背景信息

Escherichia coli contains two major aconitases (Acns), AcnA and AcnB. They are distantly related monomeric Fe-S proteins that contain different arrangements of four structural domains. acnA is specifically subject to SoxRS-mediated activation, whereas acnB encodes the major aconitase that is synthesized earlier in the growth cycle than AcnA. It is concluded that AcnB is the major citric acid cycle enzyme. Aconitate hydratase 2 (acnB) catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate, thus it functions as the major citric-acid-cycle enzyme during exponential growth. Escherichia coli acnB serves as either an enzymic catalyst or a mRNA-binding post-transcriptional regulator, depending on the status of its iron sulfur cluster. AcnB represents a large, distinct group of Gram-negative bacterial aconitases that have an altered domain organization relative to mitochondrial aconitase and other aconitases.
参考文献
  • Bradbury AJ. et al., 1996, 142 (Pt 2): 389-400.
  • Cunningham L. et al., 1997, Microbiology. 143 (Pt 12): 3795-805.
  • Jordan PA. et al., 1999, Biochem J. 344 Pt 3: 739-46.
  • Williams CH. et al., 2002, Nat Struct Biol. 9(6): 447-52.
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