Alpha-fetoprotein ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT12177
Alpha-fetoprotein ELISA Kit, Human: 产品信息
产品名称
Alpha-fetoprotein ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
5.59 pg/mL
线性范围
15.63-1000 pg/mL
特异性
Recognizes both recombinant and natural Human Alpha-fetoprotein
干扰
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 63.95 | 121.41 | 271.84 |
| SD | 4.67 | 7.31 | 13.54 |
| CV(%) | 7.3% | 6.0% | 5.0% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 105.20 | 211.97 | 419.59 |
| SD | 13.40 | 2.83 | 19.70 |
| CV(%) | 12.7% | 1.3% | 4.7% |
回收率
The recovery of Human AFP spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| serum (n=3) |
| Average % Recovery |
| 90 |
| Range |
| 85-94% |
线性关系
Serum
| Recovery of detected | |
|---|---|
| 1:2 | 114% |
| 1:4 | 104% |
| 1:8 | 83% |
| 1:16 | 83% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Alpha-fetoprotein ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Alpha-fetoprotein protein in Serum . It contains recombinant Human Alpha-fetoprotein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
Alpha-fetoprotein ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human AFP. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.
Preparations of Leptin at 20 ng/mL in a mid-range recombinant human AFP control were assayed for interference. No significant interference was observed.
Alpha-fetoprotein ELISA Kit, Human: 别称
AFPD ELISA Kit, Human; Alpha-fetoprotein ELISA Kit, Human; FETA ELISA Kit, Human; HPAFP ELISA Kit, Human
Alpha-fetoprotein 背景信息
Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitamin D (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is one of the several embryo-specific proteins and is a dominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver(1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignant growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).
全称
alpha-fetoprotein
参考文献
- Mizejewski GJ. (2001) Alpha-fetoprotein Structure and Function: Relevance to Isoforms, Epitopes, and Conformational Variants. Exp Biol Med. 226(5): 377-408.
- Tomasi TB, et al. (1977) Structure and Function of Alpha-Fetoprotein. Annual Review of Medicine. 28: 453-65.
- Leguy MC, et al. (2011) Assessment of AFP in amniotic fluid: comparison of three automated techniques. Ann Biol Clin. 69(4): 441-6.
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Rapid, quantitative and ultra-sensitive detection of cancer biomarker by a SERRS-based lateral flow immunoassay using bovine serum albumin coated Au nanorodsAuthorLu, L;Yu, J;Liu, X;Yang, X;Zhou, Z;Jin, Q;Xiao, R;Wang, C;Year2020JournalRSC Adv.ApplicationELISA
