Angiopoietin-2 ELISA Kit, Human

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Angiopoietin-2 ELISA Kit, Human: 产品信息

产品名称
Angiopoietin-2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Plasma
灵敏度
49.41 pg/mL
线性范围
78.13-5000 pg/mL
特异性
Recognizes both recombinant and natural Human Angiopoietin-2
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human Ang2 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 866.97 1,702.54 2,977.58
SD 53.82 77.59 150.71
CV(%) 6.2% 4.6% 5.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 911.25 1,808.70 3,085.10
SD 38.38 92.60 110.40
CV(%) 4.2% 5.1% 3.6%
回收率
The recovery of Human ANGPT2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
93
Range
87 -103%
Sample
plasma (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:2 105%
1:4 95%
1:8 92%
1:16 110%
Plasma
  Recovery of detected
1:2 99%
1:4 101%
1:8 95%
1:16 104%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Angiopoietin-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Angiopoietin-2 protein in Serum,Plasma . It contains recombinant Human Angiopoietin-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Angiopoietin-2 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human Ang2. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Angiopoietin-2 ELISA Kit, Human: 别称

AGPT2 ELISA Kit, Human; ANG2 ELISA Kit, Human; Angiopoietin-2 ELISA Kit, Human

Angiopoietin-2 背景信息

Angiopoietin-2 (ANG 2, or ANGPT2), is a member of the ANG family, which plays an important role in angiogenesis during the development and growth of human cancers. Both ANGPT-1 and ANGPT-2 appear to bind to the tyrosine kinase receptor, Tie-2, found primarily on the luminal surface of endothelial cells. ANG-2's role in angiogenesis generally is considered as an antagonist for ANG1, inhibiting ANG1-promoted Tie2 signaling, which is critical for blood vessel maturation and stabilization. ANG-2 modulates angiogenesis in a cooperative manner with another important angiogenic factor, vascular endothelial growth factor A. Genetic studies have revealed that ANG-2 also is critical in lymphangiogenesis during development. ANG-2 has multiple physiologic effects that regulate vascular tone, hormone secretion, tissue growth and neural activity. Several reports indicate that ANG-2 can induce neovascularization in experimental systems due to the expression of different growth factors such as angiopoietin 2, vascular endothelial factor, and its receptor, fibroblast growth factor, platelet derived growth factor, transforming growth factor beta and epidermal growth factor. In addition, ANG-2 is strongly expressed in the vasculature of many tumors and it has been suggested that ANG-2 may act synergistically with other cytokines such as vascular endothelial growth factor to promote tumor-associated Angiogenesis and tumor progression.
全称
angiopoietin 2
研究领域
参考文献
  • Thomas M, et al. (2009) The role of the Angiopoietins in vascular morphogenesis. Angiogenesis. 12(2): 125-37.
  • Hu B, et al. (2009) Angiopoietin-2: development of inhibitors for cancer therapy. Curr Oncol Rep. 11(2): 111-6.
  • Fiedler U, et al. (2006) Angiopoietins: a link between angiogenesis and inflammation. Trends Immunol. 27: 552-8.
  • Escobar E, et al. (2004) Angiotensin II, cell proliferation and angiogenesis regulator: biologic and therapeutic implications in cancer. Curr Vasc Pharmacol. 2(4): 385-99.
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