Asialoglycoprotein Receptor ELISA Kit, Human

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Asialoglycoprotein Receptor ELISA Kit, Human: 产品信息

产品名称
Asialoglycoprotein Receptor ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
38.6 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Human Asialoglycoprotein Receptor
干扰
Preparations of human CANX, NLK and APP at 40 ng/mL in a mid-range recombinant human ASGR1 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 630.38 1,279.19 2,494.53
SD 17.82 30.00 47.13
CV(%) 2.8% 2.3% 1.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 770.04 1,469.86 2,794.67
SD 49.58 91.08 182.35
CV(%) 6.4% 6.2% 6.5%
回收率
The recovery of Human ASGR1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
0.81141872513646
Range
78%-83%
Sample
supernatant (n=3)
Average % Recovery
0.928022943843094
Range
91%-95%
线性关系
Serum
  Recovery of detected
1:2 101%
1:4 92%
1:8 78%
Supernatant
  Recovery of detected
1:2 105%
1:4 100%
1:8 89%
产品概述
This Asialoglycoprotein Receptor ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Asialoglycoprotein Receptor protein in Serum,Cell supernatant . It contains recombinant Human Asialoglycoprotein Receptor, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Asialoglycoprotein Receptor ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human ASGR1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Asialoglycoprotein Receptor ELISA Kit, Human: 别称

ASGPR ELISA Kit, Human; ASGPR1 ELISA Kit, Human; CLEC4H1 ELISA Kit, Human; HL-1 ELISA Kit, Human

Asialoglycoprotein Receptor 背景信息

The asialoglycoprotein receptor (ASGPR), an endocytotic cell surface receptor expressed by hepatocytes, is triggered by triantennary binding to galactose residues of macromolecules such as asialoorosomucoid (ASOR). ASGPR belongs to the long-form subfamily of the C-type/Ca2+ dependent lectin family. It is a complex of two noncovalently-linked and highly homologous subunits, a major 42 kDa glycoprotein ASGPR1(MHL-1) and a minor 51 kDa glycoprotein ASGR2 (MHL-2). ASGPR1 is synthesized as a type II transmembrane protein that contains a cytosolic N-terminal domain, a single transmembrane segment, and an extracellular domain which contains two important structural regions. The first is a stalk domain that contributes to noncovalent oligomerization, and the second is a Ca2+-dependent carbohydrate binding domain at the very C-terminus that is unusually stabilized by three ions. The research regarded that ASGPR1 could be targeted for anti- hepatitis B virus (HBV) drug development.
全称
asialoglycoprotein receptor 1
参考文献
  • Yang J, et al. (2006) Antisense oligonucleotides targeted against asialoglycoprotein receptor 1 block human hepatitis B virus replication. J Viral Hepat. 13(3): 158-65.
  • Li Y, et al. (2008) Targeted delivery of macromolecular drugs: asialoglycoprotein receptor (ASGPR) expression by selected hepatoma cell lines used in antiviral drug development. Curr Drug Deliv. 5(4): 299-302.
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