Azurocidin/CAP37 ELISA Kit, Human

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Azurocidin/CAP37 ELISA Kit, Human: 产品信息

产品名称
Azurocidin/CAP37 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant,Plasma
灵敏度
8.76 pg/mL
线性范围
12.5-800 pg/mL
特异性
Recognizes both recombinant and natural Human Azurocidin/CAP37
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 128.84 271.84 569.30
SD 3.16 9.53 23.41
CV(%) 2.4% 3.5% 4.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 179.84 357.57 767.68
SD 12.98 26.15 41.91
CV(%) 7.2% 7.3% 5.5%
回收率
The recovery of Human AZU1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
98
Range
93-103%
Sample
supernatant (n=3)
Average % Recovery
112
Range
108-113%
Sample
plasma (n=3)
Average % Recovery
98
Range
93-100%
线性关系
Serum
  Recovery of detected
1:2 91%
1:4 109%
1:8 93%
1:16 71%
Supernatant
  Recovery of detected
1:2 101%
1:4 99%
1:8 103%
1:16 91%
Plasma
  Recovery of detected
1:2 90%
1:4 106%
1:8 94%
1:16 86%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Azurocidin/CAP37 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human Azurocidin/CAP37 protein in Serum,Cell supernatant,Plasma . It contains recombinant Human Azurocidin/CAP37, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Azurocidin/CAP37 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human AZU1. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Azurocidin/CAP37 ELISA Kit, Human: 别称

AZAMP ELISA Kit, Human; AZU ELISA Kit, Human; AZU1 ELISA Kit, Human; CAP37 ELISA Kit, Human; HBP ELISA Kit, Human; hHBP ELISA Kit, Human; HUMAZUR ELISA Kit, Human; NAZC ELISA Kit, Human

Azurocidin/CAP37 背景信息

Azurocidin (AZU1), also known as heparin-binding protein (HBP) or cationic antimicrobial protein 37 (CAP37), is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. The Azurophil granules, specialized lysosomes of the neutrophil, contain at least 1 proteins implicated in the killing of microorganisms. Azurocidin is a member of the serine protease family that includes Cathepsin G, neutrophil elastase (NE), and proteinase 3 (PR3), however, Azurocidin is not a serine proteinase since the active site serine and histidine residues are replaced. Neutrophils arriving first at sites of inflammation release Azurocidin, which acts in a paracrine fashion on endothelial cells causing the development of intercellular gaps and allowing leukocyte extravasation. It thus be regarded as a reasonable therapeutic target for a variety of inflammatory disease conditions.
全称
azurocidin 1
参考文献
  • Lindmark A, et al. (1999) Characterization of the biosynthesis, processing, and sorting of human HBP/CAP37/azurocidin. J Leukoc Biol. 66(4): 634-43.
  • Heinzelmann M, et al. (2001) Heparin binding protein (CAP37) differentially modulates endotoxin-induced cytokine production. Int J Surg Investig. 2(6): 457-66.
  • Soehnlein O, et al. (2005) Neutrophil-derived heparin-binding protein (HBP/CAP37) deposited on endothelium enhances monocyte arrest under flow conditions. J Immunol. 174(10): 6399-405.
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