Beta-2 microglobulin ELISA Kit, Rat

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Beta-2 microglobulin ELISA Kit, Rat: 产品信息

产品名称
Beta-2 microglobulin ELISA Kit, Rat
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Cell supernatant
灵敏度
1.56 pg/mL
线性范围
3.13-200 pg/mL
特异性
Recognizes both recombinant and natural Rat Beta-2 microglobulin
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant rat B2M control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 24.00 51.00 107.00
SD 1.01 2.12 4.37
CV(%) 4.2% 4.1% 4.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 24.00 50.00 103.00
SD 2.52 2.65 6.35
CV(%) 10.3% 5.3% 6.1%
回收率
The recovery of Rat B2M spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
114
Range
111 -117%
Sample
supernatant (n=3)
Average % Recovery
102
Range
101 -104%
线性关系
Serum
  Recovery of detected
1:2 114%
1:4 99%
1:8 97%
1:16 91%
Supernatant
  Recovery of detected
1:2 100%
1:4 100%
1:8 93%
1:16 89%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Beta-2 microglobulin ELISA Kit, Rat is an enzyme-linked immunosorbent assay for the quantitative measurement of Rat Beta-2 microglobulin protein in Serum,Cell supernatant . It contains recombinant Rat Beta-2 microglobulin, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Beta-2 microglobulin ELISA Kit, Rat: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural rat B2M. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

Beta-2 microglobulin 背景信息

B2M, also known as β2-Microglobulin or CDABP92, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M(β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M(β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens.B2M(β2- Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to theα3 chain. Unlikeα3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M(β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M(β2 microglobulin) can indicate non-progression of HIV.
全称
beta-2 microglobulin
参考文献
  • Poulik MD, et al. (1979) Beta 2-Microglobulin: methods and clinical applications. CRC Ctit Rev Clin Lab Sci. 10(3): 225-45.
  • Poulik MD, et al. (1975) Beta2-Microglobulins. Contemp Top Mol Immunol. 4: 157-204.
  • Berggard I. (1976) Beta2-Microglobulins: isolation, properties, and distribution. Fed Proc. 35(5): 1167-70.
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