B4GALT1 ELISA Kit, Human

Temporarily not available outside of China.The online Datasheet here is only a general version, for batch specific information please refer to the hard copy included in the product shipping package.

B4GALT1 ELISA Kit, Human: 产品信息

产品名称
B4GALT1 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
78.00 pg/mL
线性范围
156.25-10000 pg/mL
特异性
Recognizes both recombinant and natural Human B4GALT1
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.94 3.89 7.88
SD 0.06 0.12 0.39
CV(%) 3.2% 3.1% 5.0%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1.57 3.02 6.28
SD 0.10 0.23 0.58
CV(%) 6.3% 7.5% 9.3%
回收率
The recovery of Human B4GALT1 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
108
Range
105-115%
线性关系
Supernatant
  Recovery of detected
1:2 109%
1:4 108%
1:8 106%
1:16 96%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This B4GALT1 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human B4GALT1 protein in Lysate . It contains recombinant Human B4GALT1, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

B4GALT1 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human B4GALT1.The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

B4GALT1 ELISA Kit, Human: 别称

B4GAL-T1 ELISA Kit, Human; beta4Gal-T1 ELISA Kit, Human; CDG2D ELISA Kit, Human; GGTB2 ELISA Kit, Human; GT1 ELISA Kit, Human; GTB ELISA Kit, Human

B4GALT1 背景信息

Beta1,4-Galactosyltransferase-I (B4GALT1), one of seven beta1,4-galactosyltransferases, is an enzyme commonly found in the trans-Golgi complex that adds galactose to oligosaccharides. They have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. B4GALT1 gene directs production of B4GALT1 protein using either of two transcription start sites. The product of the smaller transcript serves the traditional biosynthetic role in the Golgi. This form also complexes with α-lactalbumin, a mammary-specific protein, to form lactose synthase. In addition to a biosynthetic role, the protein translated from the longer transcript appears on the plasma membranes of some cells where it serves as a signalling receptor in cell-matrix interactions such as sperm-egg binding.
全称
UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1
参考文献
  • Hennet T. (2002) The galactosyltransferase family. Cellular and Molecular Life Sciences. 59(7): 1081-95.
  • Landers EA, et al. (2009) Porcine 1, 4-Galactosyltransferase-I Sequence and Expression. Reproduction in Domestic Animals. 44(2): 228-34.
  • Amado M, et al. (2000) Identification and characterization of large galactosyltransferase gene families: galactosyltransferases for all functions. Biochim Biophys Acta. 1473 (1): 35-53.
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