B7-H3 ELISA Kit, Human

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B7-H3 ELISA Kit, Human: 产品信息

产品名称
B7-H3 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Urine
灵敏度
16.20 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Human B7-H3
干扰
Preparations of human B7-1, B7-2 and PD-L2 at 50 ng/mL in a mid-range recombinant human B7H3 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 264.28 490.13 932.43
SD 6.32 12.00 25.22
CV(%) 2.4% 2.4% 2.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 272.45 547.73 1,022.38
SD 13.81 23.71 32.98
CV(%) 5.1% 4.3% 3.2%
回收率
The recovery of Human CD276 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
86
Range
80 -90%
Sample
urine (n=3)
Average % Recovery
107
Range
106 -109%
线性关系
Serum
  Recovery of detected
1:2 109%
1:4 130%
1:8 123%
1:16 107%
Urine
  Recovery of detected
1:2 92%
1:4 108%
1:8 118%
1:16 99%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This B7-H3 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human B7-H3 protein in Serum,Urine . It contains recombinant Human B7-H3, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

B7-H3 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human B7H3. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

B7-H3 ELISA Kit, Human: 别称

4Ig-B7-H3 ELISA Kit, Human; B7-H3 ELISA Kit, Human; B7H3 ELISA Kit, Human; B7RP-2 ELISA Kit, Human

B7-H3 背景信息

B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.
全称
CD276 molecule
研究领域
参考文献
  • Suh WK, et al. (2004) The immune regulatory protein B7-H3 promotes osteoblast differentiation and bone mineralization. Proc Natl Acad Sci U S A. 101(35): 12969-73.
  • Waschbisch A, et al. (2008) Human muscle cells express the costimulatory molecule B7-H3, which modulates muscle-immune interactions. Arthritis Rheum. 58(11): 3600-8.
  • Loos M, et al. (2010) B7-h3 and its role in antitumor immunity. Clin Dev Immunol. 2010: 683875.
  • Zang X, et al. (2010) Tumor associated endothelial expression of B7-H3 predicts survival in ovarian carcinomas. Mod Pathol. 23(8): 1104-12.
  • Sun J, et al. (2010) Clinical significance and regulation of the costimulatory molecule B7-H3 in human colorectal carcinoma. Cancer Immunol Immunother. 59(8): 1163-71.
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