BCAM ELISA Kit, Human

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BCAM ELISA Kit, Human: 产品信息

产品名称
BCAM ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
17.7 pg/mL
线性范围
39-2500 pg/mL
特异性
Recognizes both recombinant and natural Human BCAM
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.44 0.75 1.46
SD 0.03 0.04 0.08
CV(%) 5.9% 5.1% 5.4%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.38 0.74 1.44
SD 0.04 0.02 0.04
CV(%) 9.4% 2.8% 3.0%
回收率
The recovery of Human BCAM spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
95.7593482348026
Range
87%-102%
线性关系
Serum
  Recovery of detected
1:2 111%
1:4 98%
1:8 98%
1:16 127%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This BCAM ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human BCAM protein in Serum . It contains recombinant Human BCAM, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

BCAM ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human BCAM. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

BCAM ELISA Kit, Human: 别称

AU ELISA Kit, Human; CD239 ELISA Kit, Human; LU ELISA Kit, Human; MSK19 ELISA Kit, Human

BCAM 背景信息

The Lutheran (Lu) blood group and basal cell adhesion molecule (BCAM) antigens are both carried by 2 glycoprotein isoforms of the immunoglobulin superfamily representing receptors for the laminin alpha(5) chain. It is a transmembrane receptor with five immunoglobulin-like domains in its extracellular region, and is therefore classified as a member of the immunoglobulin (Ig) gene family. In addition to red blood cells, Lu/BCAM proteins are expressed in endothelial cells of vascular capillaries and in epithelial cells of several tissues. BCAM/LU has a wide tissue distribution with a predominant expression in the basal layer of the epithelium and the endothelium of blood vessel walls. As designated as CD239 recently, BCAM and LU share a significant sequence similarity with the CD146 (MUC18) and CD166, and themselves are adhesion molecules that bind laminin with high affinity. Laminins are found in all basement membranes and are involved in cell differentiation, adhesion, migration, and proliferation. BCAM is upregulated following malignant transformation of some cell types in vivo and in vitro, thus being a candidate molecule involved in tumor progression. In addition, BCAM interacts with integrin in sickle red cells, and thus may potentially play a role in vaso-occlusive episodes.
全称
basal cell adhesion molecule (Lutheran blood group)
参考文献
  • Kikkawa Y, et al. (2005) Review: Lutheran/B-CAM: a laminin receptor on red blood cells and in various tissues. Connect Tissue Res. 46 (4-5): 193-9.
  • El Nemer W, et al. (2007) Endothelial Lu/BCAM glycoproteins are novel ligands for red blood cell alpha4beta1 integrin: role in adhesion of sickle red blood cells to endothelial cells. Blood. 109 (8): 3544-51.
  • Colin Y, et al. (2008) Red cell and endothelial Lu/BCAM beyond sickle cell disease. Transfus Clin Biol. 15 (6): 402-5.
  • El Nemer W, et al. (2008) Role of Lu/BCAM in abnormal adhesion of sickle red blood cells to vascular endothelium. Transfus Clin Biol. 15 (1-2): 29-33.
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