BID ELISA Kit, Human

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BID ELISA Kit, Human: 产品信息

产品名称
BID ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
4.57 pg/mL
线性范围
15.63-1000 pg/mL
特异性
Recognizes both recombinant and natural Human BID
干扰
Preparations of human APP at 20 ng/mL in a mid-range recombinant human BID control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 98.12 201.23 409.48
SD 6.81 12.31 9.45
CV(%) 6.9% 6.1% 2.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 152.80 322.20 596.40
SD 8.96 14.99 13.63
CV(%) 5.9% 4.7% 2.3%
回收率
The recovery of Human BID spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=4)
Average % Recovery
93
Range
86-109%
线性关系
Serum
  Recovery of detected
1:2 104%
1:4 109%
1:8 113%
1:16 122%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This BID ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human BID protein in Serum . It contains recombinant Human BID, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

BID ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human BID. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

BID ELISA Kit, Human: 别称

FP497 ELISA Kit, Human

BID 背景信息

The BH3 interacting domain death agonist (BID) is a pro-apoptotic member of the Bcl-2 protein family, which contains only the BH3 domain, and is required for its interaction with the Bcl-2 family proteins and for its pro-death activity. BID is important to cell death mediated by these proteases and thus is the sentinel to protease-mediated death signals. Recent studies further indicate that Bid may be more than just a killer molecule, it could be also involved in the maintenance of genomic stability by engaging at mitosis checkpoint. BID is an integrating key regulator of the intrinsic death pathway that amplifies caspase-dependent and caspase-independent execution of neuronal apoptosis. Therefore pharmacological inhibition of BID provides a promising therapeutic strategy in neurological diseases where programmed cell death is prominent. BID is activated by Caspase 8 in response to Fas/TNF-R1 death receptor activation. Activated BID is translocated to mitochondria and induces cytochrome c release, which in turn activates downstream caspases. BID action has been proposed to involve the mitochondrial re-location of its truncated form, tBid, to facilitate the release of apoptogenic proteins like cytochrome c.
全称
BH3 interacting domain death agonist
Related Pathways
  • Death Receptor Signaling
    Death Receptor Signaling
  • TNF Signaling
    TNF Signaling
参考文献
  • Gross A. (2006) BID as a double agent in cell life and death. Cell Cycle. 5(6): 582-4.
  • Yin XM. (2007) Bid, a BH3-only multi-functional molecule, is at the cross road of life and death. Gene. 369: 7-19.
  • Esposti MD. (2002) The roles of Bid. Apoptosis. 7(5): 433-40.
  • Yin XM. (2000) Signal transduction mediated by Bid, a pro-death Bcl-2 family proteins, connects the death receptor and mitochondria apoptosis pathways. Cell Res. 10(3): 161-7.
  • Yin XM. (2000) Bid, a critical mediator for apoptosis induced by the activation of Fas/TNF-R1 death receptors in hepatocytes. J Mol Med. 78(4): 203-11.
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