BMP-2 ELISA Kit, Human
Temporarily not available outside of China. The substitute is ELISA Pair Set (Cat# SEK10426).
Cat: KIT10426
BMP-2 ELISA Kit, Human: 产品信息
产品名称
BMP-2 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
17.25 pg/mL
线性范围
62.5-2500 pg/mL
特异性
Recognizes both recombinant and natural Human BMP-2
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 258.00 | 518.00 | 971.00 |
| SD | 7.58 | 11.74 | 30.18 |
| CV(%) | 3.0% | 2.3% | 3.1% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 216.00 | 461.00 | 852.00 |
| SD | 23.62 | 35.25 | 88.90 |
| CV(%) | 11.0% | 7.6% | 10.4% |
回收率
The recovery of Human BMP2 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| supernatant (n=3) |
| Average % Recovery |
| 78 |
| Range |
| 76 -80% |
线性关系
Supernatant
| Recovery of detected | |
|---|---|
| 1:2 | 99% |
| 1:4 | 104% |
| 1:8 | 96% |
| 1:16 | 81% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This BMP-2 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human BMP-2 protein in Recombinant protein . It contains recombinant Human BMP-2, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
BMP-2 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Human BMP2. The factors listed below were prepared at 50 or 100 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
BMP-2 ELISA Kit, Human: 别称
BDA2 ELISA Kit, Human; BDA2A ELISA Kit, Human; BMP-2 ELISA Kit, Human; BMP2A ELISA Kit, Human
BMP-2 背景信息
BMP-2 protein, like other bone morphogenetic proteins, plays an important role in the development of bone and cartilage. BMP-2 protein is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction. BMP-2 and BMP-7 are osteogenic BMPs that have been demonstrated to potently induce osteoblast differentiation in a variety of cell types. BMP-2, BMP-4 and BMP-7 are known to be of major importance in bone formation and repair. In cancerous tissues BMP-2 protein may play an important role in the progression of glioma.
全称
bone morphogenetic protein 2
参考文献
- Jiao X, et al. (2007) Heparan sulfate proteoglycans (HSPGs) modulate BMP-2 osteogenic bioactivity in C2C12 cells. J Biol Chem. 282(2):1080-6.
- Michon F, et al. (2008) BMP-2 and BMP-7 play antagonistic roles in feather induction. Development 135 (16):2797-805.
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