c-MET ELISA Kit, Human

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c-MET ELISA Kit, Human: 产品信息

产品名称
c-MET ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Plasma
灵敏度
33.18 pg/mL
线性范围
101.56-6500 pg/mL
特异性
Recognizes both recombinant and natural Human c-MET
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human HGFR control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,110.00 2,277.00 4,129.00
SD 58.42 125.11 211.93
CV(%) 5.3% 5.5% 5.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,114.00 2,300.00 4,316.00
SD 37.69 40.13 184.09
CV(%) 3.4% 1.7% 4.3%
回收率
The recovery of Human MET spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
119
Range
109 -125%
Sample
plasma (n=3)
Average % Recovery
89
Range
82 -92%
线性关系
Serum
  Recovery of detected
1:2 98%
1:4 102%
1:8 91%
1:16 73%
Plasma
  Recovery of detected
1:2 96%
1:4 104%
1:8 109%
1:16 100%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This c-MET ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human c-MET protein in Serum,Plasma . It contains recombinant Human c-MET, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

c-MET ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human HGFR. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

c-MET ELISA Kit, Human: 别称

AUTS9 ELISA Kit, Human; c-Met ELISA Kit, Human; DFNB97 ELISA Kit, Human; HGFR ELISA Kit, Human; RCCP2 ELISA Kit, Human

c-MET 背景信息

Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.
全称
MET proto-oncogene, receptor tyrosine kinase
参考文献
  • McGill GG, et al. (2006) c-Met expression is regulated by Mitf in the melanocyte lineage. J Biol Chem. 281(15): 10365-73.
  • Garcia S, et al. (2007) c-Met overexpression in inflammatory breast carcinomas: automated quantification on tissue microarrays. British journal of cancer. 96(2): 329-35.
  • Socoteanu MP, et al. (2008) c-Met targeted therapy of cholangiocarcinoma. World J Gastroenterol. 14(19): 2990-4.
  • Kong DS, et al. (2009) Prognostic significance of c-Met expression in glioblastomas. Cancer. 115(1): 140-8.
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