C-Reactive Protein ELISA Kit, Human

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C-Reactive Protein ELISA Kit, Human: 产品信息

产品名称
C-Reactive Protein ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
21.78 pg/mL
线性范围
54.69-3500 pg/mL
特异性
Recognizes both recombinant and natural Human C-Reactive Protein
干扰
Preparations of the factors listed below at 50 ng/mL in a mid-range recombinant human CRP control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 516.00 1,012.00 2,061.00
SD 16.29 38.75 100.06
CV(%) 3.2% 3.8% 4.9%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 520.00 1,039.00 2,047.00
SD 14.45 29.47 19.24
CV(%) 2.8% 2.8% 0.9%
回收率
The recovery of Human CRP spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
101
Range
99 -104%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Serum
  Recovery of detected
1:1 98%
1:2 98%
1:4 102%
1:8 102%
Supernatant
  Recovery of detected
1:1 93%
1:2 98%
1:4 109%
1:8 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This C-Reactive Protein ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human C-Reactive Protein protein in Serum . It contains recombinant Human C-Reactive Protein, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

C-Reactive Protein ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CRP. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

C-Reactive Protein ELISA Kit, Human: 别称

C-Reactive Protein ELISA Kit, Human; PTX1 ELISA Kit, Human

C-Reactive Protein 背景信息

C-reactive protein (CRP) is synthesized by the liver in response to factors released by fat cells. It is a member of the pentraxin family of proteins. The levels of CRP rise in response to inflammation. Human C-reactive protein (CRP) is the classical acute phase reactant, the circulating concentration of which rises rapidly and extensively in a cytokine-mediated response to tissue injury, infection and inflammation. Serum CRP values are routinely measured, empirically, to detect and monitor many human diseases. However, CRP is likely to have important host defence, scavenging and metabolic functions through its capacity for calcium-dependent binding to exogenous and autologous molecules containing phosphocholine (PC) and then activating the classical complement pathway. CRP may also have pathogenic effects and the recent discovery of a prognostic association between increased CRP production and coronary atherothrombotic events is of particular interest.
全称
C-reactive protein, pentraxin-related
参考文献
  • Pepys MB. et al., 2003, J Clin Invest. 111 (12): 1805-12.
  • Thompson D. et al., 1999, Structure. 7(2): 169-77.
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