Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse

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Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse: 产品信息

产品名称
Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
13.91 pg/mL
线性范围
31.25-2000 pg/mL
特异性
Recognizes both recombinant and natural Mouse Carbonic Anhydrase 8/CA8
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 366.00 740.00 1,508.00
SD 5.45 10.85 23.33
CV(%) 1.5% 1.5% 1.5%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 334.00 704.00 1,454.00
SD 30.24 28.13 58.57
CV(%) 9.1% 4.0% 4.0%
回收率
The recovery of Mouse CA8 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
72
Range
71-75%
线性关系
Supernatant
  Recovery of detected
1:2 93%
1:4 102%
1:8 113%
1:16 115%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Carbonic Anhydrase 8/CA8 protein in Recombinant protein . It contains recombinant Mouse Carbonic Anhydrase 8/CA8, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant Mouse CA8. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Cross-reactivity was observed to be 7% with 50 ng/mL of recombinant human CA8.

Carbonic Anhydrase 8/CA8 ELISA Kit, Mouse: 别称

AW546993 ELISA Kit, Mouse; Ca8 ELISA Kit, Mouse; Cals ELISA Kit, Mouse; Cals1 ELISA Kit, Mouse; Car8 ELISA Kit, Mouse; Carp ELISA Kit, Mouse; RP23-180H12.1 ELISA Kit, Mouse; wdl ELISA Kit, Mouse

Carbonic Anhydrase 8/CA8 背景信息

The carbonic anhydrases (or carbonate dehydratases) are classified as metalloenzyme for its zinc ion prosthetic group and form a family of enzymes that catalyze the rapid interconversion of carbon dioxide and water to bicarbonate and protons, a reversible reaction that takes part in maintaining acid-base balance in blood and other tissues. The carbonic anhydrasekl (CA) family consists of at least 11 enzymatically active members and a few inactive homologous proteins. Carbonic anhydrase protein (CA) VIII, which is a member of the CA gene family, has been shown to have no catalytic CA activity and its biological function is still unknown. Increased expression of CA-RP VIII was observed in 78% of colorectal carcinomas. It suggested that CA-RP VIII plays a role in the process of invasion in colorectal cancer.
全称
carbonic anhydrase VIII
参考文献
  • Lehtonen J, et al. (2004) Characterization of CA XIII, a Novel Member of the Carbonic Anhydrase Isozyme Family. The Journal of Biological Chemistry. 279: 2719-27.
  • Lindskog S. (1997) Structure and mechanism of carbonic anhydrase. Pharmacology & Therapeutics. 74(1):1-20.
  • Miyaji E, et al. (2003) Overexpression of carbonic anhydrase-related protein VIII in human colorectal cancer. The Journal of Pathology. 201(1): 37-45.
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