CALML5 ELISA Kit, Human

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CALML5 ELISA Kit, Human: 产品信息

产品名称
CALML5 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Recombinant protein
灵敏度
4.1 pg/mL
线性范围
23.44-1500 pg/mL
特异性
Recognizes both recombinant and natural Human CALML5
干扰
Preparations of PLK1 at 15 ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.24 0.49 0.97
SD 0.01 0.01 0.03
CV(%) 4.8% 2.6% 2.8%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 0.22 0.44 0.84
SD 0.02 0.04 0.03
CV(%) 7.0% 9.6% 3.5%
回收率
The recovery of Human CALML5 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
supernatant (n=4)
Average % Recovery
111
Range
100-120%
线性关系
Supernatant
  Recovery of detected
1:2 113%
1:4 115%
1:8 90%
1:16 92%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CALML5 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CALML5 protein in Recombinant protein . It contains recombinant Human CALML5, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CALML5 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes recombinant human CALML5. The factors listed above were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
Preparations of PLK1 at 15 ng/mL in a mid-range recombinant human CALML5 control were assayed for interference. No significant interference was observed.

CALML5 ELISA Kit, Human: 别称

CLSP ELISA Kit, Human

CALML5 背景信息

Calmodulin-like protein 5, also known as Calmodulin-like skin protein, CALML5 and CLSP, is a protein which contains fourEF-hand domains. CALML5 / CLSP is particularly abundant in the epidermis where its expression is directly related to keratinocyte differentiation.The expression is very low in lung. CALML5 / CLSP binds calcium. It may be involved in terminal differentiation of keratinocytes. Coxsackievirus and adenovirus receptor (CAR) is a member of the immunoglobulin (Ig) superfamily and a component of epithelial tight junction. CAR functions as a primary receptor for coxsackievirus B and adenovirus (Ad) infection. CALML5 / CLSP is closely related to CAR. The structure and dynamics of human calmodulin-like skin protein CALML5 / CLSP have been characterized by NMR spectroscopy. The mobility of CALML5 / CLSP has been found to be different for the N-terminal and C-terminal domains. The N-terminal domain is characterized by four stable helices, which experience large fluctuations. This is shown to be due to mutations in the hydrophobic core. The overall N-terminal domain behavior is similar both in the full-length protein and in the isolated domain.
全称
calmodulin like 5
Related Pathways
  • Actin Dynamics Signaling Pathway
    Actin Dynamics Signaling Pathway
  • B Cell Receptor Signaling Pathway
    B Cell Receptor Signaling Pathway
  • T Cell Receptor Signaling Pathway
    T Cell Receptor Signaling Pathway
参考文献
  • Mehul B., et al., 2000, J. Biol. Chem. 275:12841-12847.
  • Babini E., et al., 2006, Structure 14:1029-1038.
  • Kawabata,K. et al., 2007, Gene Ther. 14 (16):1199-207.
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