CD147 ELISA Kit, Human

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CD147 ELISA Kit, Human: 产品信息

产品名称
CD147 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum,Urine
灵敏度
3.45 pg/mL
线性范围
7.81-500 pg/mL
特异性
Recognizes both recombinant and natural Human CD147
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 102.00 199.90 383.50
SD 6.35 8.83 12.04
CV(%) 6.2% 4.4% 3.1%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 90.50 180.10 360.10
SD 7.98 16.36 39.81
CV(%) 8.8% 9.1% 11.1%
回收率
The recovery of Human BSG spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
102
Range
98 -106%
Sample
urine (n=3)
Average % Recovery
88
Range
78 -96%
线性关系
Serum
  Recovery of detected
1:2 91%
1:4 105%
1:8 102%
1:16 102%
Urine
  Recovery of detected
1:2 96%
1:4 108%
1:8 102%
1:16 92%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD147 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD147 protein in Serum,Urine . It contains recombinant Human CD147, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD147 ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD147. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed. Preparations of the following factors at 50 ng/mL in a mid-range recombinant human CD147 control were assayed for interference. No significant interference was observed.

CD147 ELISA Kit, Human: 别称

5F7 ELISA Kit, Human; Basigin ELISA Kit, Human; CD147 ELISA Kit, Human; EMMPRIN ELISA Kit, Human; M6 ELISA Kit, Human; OK ELISA Kit, Human; TCSF ELISA Kit, Human

CD147 背景信息

CD147/EMMPRIN (Extracellular Matrix Metalloproteinase Inducer), also known as Basigin (BSG), is a transmembrane glycoprotein with different forms resulted from different modes of glycosylation and N-terminal sequence variants. It is a member of the immunoglobulin superfamily with homology to both the immunoglobulin V domain and MHC class II antigen beta-chain. This protein play important roles in variety of events including spermatogenesis, embryo implantation, neural network formation. CD147 induces the production and release of matrix metalloproteinases (MMP) in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Furthermore, CD147 also serves as a receptor for extracellular cyclophilinthe and its association with integrins might be important in signal transduction. Recently, CD147 displays increased expression in many cancers, and it has been previously demonstrated to participate in cancer metastasis and progression. Thus, CD147 and its antibody are used as an effective treatment for malignant cancers.
全称
basigin (Ok blood group)
参考文献
  • Tang Y, et al. (2004) Tumor-stroma interaction: positive feedback regulation of extracellular matrix metalloproteinase inducer (EMMPRIN) expression and matrix metalloproteinase-dependent generation of soluble EMMPRIN. Mol Cancer Res. 2(2): 73-80.
  • Wilson MC, et al. (2005) Basigin (CD147) is the target for organomercurial inhibition of monocarboxylate transporter isoforms 1 and 4: the ancillary protein for the insensitive MCT2 is EMBIGIN (gp70). J Biol Chem. 280(29): 27213-21.
  • Curtin KD, et al. (2005) Basigin (EMMPRIN/CD147) interacts with integrin to affect cellular architecture. J Cell Sci. 118(Pt 12): 2649-60.
  • Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44. Zhu H, et al. (2009) A novel antibody fragment targeting HAb18G/CD147 with cytotoxicity and decreased immunogenicity. Cancer Biol Ther. 8(11): 1035-44.
  • Seizer P, et al. (2009) EMMPRIN (CD147) is a novel receptor for platelet GPVI and mediates platelet rolling via GPVI-EMMPRIN interaction. Thromb Haemost. 101(4): 682-6.
  • Moonsom S, et al. (2010) A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma (Larchmt). 29(1): 45-52.
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