CD40 ELISA Kit, Human
Temporarily not available outside of China.
Cat: KIT10774
CD40 ELISA Kit, Human: 产品信息
产品名称
CD40 ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Urine
灵敏度
2.55 pg/mL
线性范围
7.8-500 pg/mL
特异性
Recognizes both recombinant and natural Human CD40
精密度
| Intra-assay Precision | |||
|---|---|---|---|
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 98.00 | 219.00 | 445.00 |
| SD | 3.62 | 5.05 | 7.35 |
| CV(%) | 3.7% | 2.3% | 1.6% |
| Inter-assay Precision | |||
| Sample | 1 | 2 | 3 |
| N | 20 | 20 | 20 |
| Mean(pg/mL) | 102.00 | 214.00 | 418.00 |
| SD | 7.85 | 17.74 | 55.03 |
| CV(%) | 7.7% | 8.3% | 13.2% |
回收率
The recovery of Human CD40 spiked to different levels throughout the range of the assay in related matrices was evaluated.
| Sample |
|---|
| urine (n=3) |
| Average % Recovery |
| 100 |
| Range |
| 99 -101% |
线性关系
Urine
| Recovery of detected | |
|---|---|
| 1:2 | 80% |
| 1:4 | 98% |
| 1:8 | 102% |
| 1:16 | 102% |
ELISA 试剂盒（即用型）组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD40 ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD40 protein in Urine . It contains recombinant Human CD40, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA
Opened/Reconstituted Reagents: Please refer to CoA
CD40 ELISA Kit, Human: 图片
This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD40. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.
CD40 ELISA Kit, Human: 别称
Bp50 ELISA Kit, Human; CDW40 ELISA Kit, Human; p50 ELISA Kit, Human; TNFRSF5 ELISA Kit, Human
CD40 背景信息
CD40, also known as TNFRSF5, is a member of the TNF receptor superfamily which are single transmembrane-spanning glycoproteins. CD40 protein plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. CD40 protein is expressed in B cells, dendritic cells, macrophages, endothelial cells, and several tumor cell lines. Defects in CD40 result in hyper-IgM immunodeficiency type 3 (HIGM3). In addition, CD40/CD40L interaction is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.
- Cancer Immunotherapy
- Co-stimulatory Immune Checkpoint Targets
- Immune Checkpoint
- Immune Checkpoint Detection: Antibodies
- Immune Checkpoint Detection: ELISA Antibodies
- Immune Checkpoint Detection: FCM Antibodies
- Immune Checkpoint Detection: ICC Antibodies
- Immune Checkpoint Detection: IP Antibodies
- Immune Checkpoint Detection: WB Antibodies
- Immune Checkpoint Proteins
- Immune Checkpoint Targets
- Immunotherapy
- Targeted Therapy
全称
CD40 molecule, TNF receptor superfamily member 5
研究领域
Related Pathways
-
B Cell Receptor Signaling Pathway
参考文献
- van Kooten C, et al. (2000). CD40-CD40 ligand. J Leukoc Biol. 67 (1): 2-17.
- Bhushan A, et al. (2002). CD40:CD40L interactions in X-linked and non-X-linked hyper-IgM syndromes. Immunol Res. 24 (3): 311-24.
- Chatzigeorgiou A, et al. (2009) CD40/CD40L signaling and its implication in health and disease. Biofactors. 35(6): 474-83.
- Li R, et al. (2009) Expression of CD40 and CD40L in Gastric Cancer Tissue and Its Clinical Significance. Int J Mol Sci. 10(9): 3900-17.
- Lievens D, et al. (2009) The multi-functionality of CD40L and its receptor CD40 in atherosclerosis. Thromb Haemost. 102(2): 206-14.
