CD48 ELISA Kit, Mouse

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CD48 ELISA Kit, Mouse: 产品信息

产品名称
CD48 ELISA Kit, Mouse
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Lysate
灵敏度
16.01 pg/mL
线性范围
62.5-4000 pg/mL
特异性
Recognizes both recombinant and natural Mouse CD48
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 643.00 1,320.00 2,617.00
SD 25.43 52.44 97.33
CV(%) 4.0% 4.0% 3.7%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 729.00 1,507.00 2,956.00
SD 67.53 113.31 193.14
CV(%) 9.3% 7.5% 6.5%
回收率
The recovery of Mouse CD48 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
81 -87%
Sample
supernatant (n=3)
Average % Recovery
100
Range
91 -105%
Sample
urine (n=3)
Average % Recovery
84
Range
81 -87%
线性关系
Supernatant
  Recovery of detected
1:1 93%
1:2 98%
1:4 109%
1:8 95%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD48 ELISA Kit, Mouse is an enzyme-linked immunosorbent assay for the quantitative measurement of Mouse CD48 protein in Lysate . It contains recombinant Mouse CD48, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD48 ELISA Kit, Mouse: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Mouse CD48. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD48 ELISA Kit, Mouse: 别称

AI449234 ELISA Kit, Mouse; AW610730 ELISA Kit, Mouse; Bcm-1 ELISA Kit, Mouse; BCM1 ELISA Kit, Mouse; BLAST ELISA Kit, Mouse; BLAST-1 ELISA Kit, Mouse; BLAST1 ELISA Kit, Mouse; MEM-102 ELISA Kit, Mouse; Sgp-60 ELISA Kit, Mouse; SLAMF2 ELISA Kit, Mouse

CD48 背景信息

Cluster of Differentiation 48 (CD48), also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 protein performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.
全称
CD48 molecule
研究领域
参考文献
  • Messmer B, et al. (2006) CD48 stimulation by 2B4 (CD244)-expressing targets activates human NK cells. J Immunol. 176(8): 4646-50
  • Milstein O, et al. (2008) Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 283(49): 34414-22.
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