CD55/DAF ELISA Kit, Human

Temporarily not available outside of China.
1/2
Price:
Size:
Number:

CD55/DAF ELISA Kit, Human: 产品信息

产品名称
CD55/DAF ELISA Kit, Human
检测原理
Solid Phase Sandwich ELISA (quantitative)
偶联物
HRP
检测样品
Serum
灵敏度
91.65 pg/mL
线性范围
125-8000 pg/mL
特异性
Recognizes both recombinant and natural Human CD55/DAF
精密度
  Intra-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,915.32 3,714.11 6,592.55
SD 42.23 98.04 284.36
CV(%) 2.2% 2.6% 4.3%
  Inter-assay Precision
Sample 1 2 3
N 20 20 20
Mean(pg/mL) 1,483.40 3,332.00 6,295.20
SD 37.20 77.29 371.11
CV(%) 2.5% 2.3% 5.9%
回收率
The recovery of Human CD55 spiked to different levels throughout the range of the assay in related matrices was evaluated.
Sample
serum (n=3)
Average % Recovery
84
Range
82 -86%
线性关系
Serum
  Recovery of detected
1:2 92%
1:4 92%
1:8 89%
1:16 91%
ELISA 试剂盒(即用型)组分
1. 96 well microplate coated with Capture Antibody
2. Detection Antibody conjugated to HRP
3. Standards
4. Wash Buffer Concentrate
5. Dilution Buffer Concentrate
6. Color Reagent A
7. Color Reagent B
8. Stop Solution
产品概述
This CD55/DAF ELISA Kit, Human is an enzyme-linked immunosorbent assay for the quantitative measurement of Human CD55/DAF protein in Serum . It contains recombinant Human CD55/DAF, and antibodies raised against the recombinant protein. This ELISA kit is complete and ready-to-use.
运输方式
This ELISA Kit is shipped at ambient temperature.
储存条件
Unopened Kit: Store at 2 - 8℃
Opened/Reconstituted Reagents: Please refer to CoA

CD55/DAF ELISA Kit, Human: 图片

This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.
This assay recognizes both recombinant and natural Human CD55. The factors listed below were prepared at 50 ng/mL in dilution buffer and assayed for cross-reactivity. No cross-reactivity was observed.

CD55/DAF ELISA Kit, Human: 别称

CR ELISA Kit, Human; CROM ELISA Kit, Human; DAF ELISA Kit, Human; TC ELISA Kit, Human

CD55/DAF 背景信息

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 3 SCRs (short consensus repeats) in their plasma-exposed regions. It is a major regulator of the alternative and classical pathways of complement activation and is expressed on all serum-exposed cells. CD55 is physiologically acting as an inhibitor of the complement system, but is also broadly expressed in malignant tumours. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. It is commonly hijacked by invading pathogens, including many enteroviruses and uropathogenic Escherichia coli, to promote cellular attachment prior to infection. This 7-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It inhibits complement activation by suppressing the function of C3/C5 convertases, thereby limiting local generation or deposition of C3a/C5a and membrane attack complex (MAC or C5b-9) production. DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology.
全称
CD55 molecule, decay accelerating factor for complement (Cromer blood group)
Related Pathways
  • Complement Activation Pathways
    Complement Activation Pathways
参考文献
  • Lea S. (2002) Interactions of CD55 with non-complement ligands. Biochem Soc Trans. 30(Pt 6): 1014-9.
  • Mikesch JH, et al. (2006) The expression and action of decay-accelerating factor (CD55) in human malignancies and cancer therapy. Cell Oncol. 28(5-6): 223-32.
  • Wang Y, et al. (2010) Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury. J Neuroinflammation. 7:24.
  • The Asthma-associated PER1-like domain-containing protein 1 (PERLD1) Haplotype Influences Soluble Glycosylphosphatidylinositol Anchor Protein (sGPI-AP) Levels in Serum and Immune Cell Proliferation
    Author
    Sio, YY;Anantharaman, R;Lee, SQE;Matta, SA;Ng, YT;Chew, FT;
    Year
    2020
    Journal
    Sci Rep
添加购物车成功! 添加购物车失败!请再次尝试 正在更新购物车,请稍后 U.S.A.